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| Catalog Code: | NB120-17798 |
| Product Name: | XPF Antibody |
| Product Description: | Mouse Monoclonal anti-XPF (SPM228) |
| Clone: | SPM228 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Recombinant human XPF protein |
| Cross Reactivity: | Cross-reacts with Human. |
| Packaging: | 0.5 ml protein A purified Mouse IgG. |
| Uses: | IHC-P: 1/50. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 minWB: Use at a concentration of 1 - 2 ug/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3' of the DNA lesion while the ERCC1 / XPF complex makes the 5' incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG2 kappa |
| Host Name: | Mouse |
| Buffer: | BSA, 10mM PBS. pH 7.4 |
| Application Summary: | Western Blot , Immunohistochemistry-Paraffin |
| Species Summary: | Hu, |
| Alternate Names: | anti-DNA excision repair protein ERCC 4 antibody, anti-DNA excision repair protein ERCC4 antibody, anti-DNA repair endonuclease XPF antibody, anti-DNA repair protein complementing XP F cells antibody, anti-ERCC 11 antibody, anti-ERCC 4 antibody, anti-ERCC11 antibody, anti-ERCC4 antibody, anti-Excision repair complementing defective in Chinese hamster antibody, anti-Excision repair cross complementing rodent repair deficiency complementation group 4 antibody, anti-RAD 1 antibody, anti-RAD1 antibody, anti-Xeroderma Pigmentosum Complementation group F antibody, anti-Xeroderma pigmentosum group F complementing protein antibody |
| Package Size: | 0.5 ml |
| Preservative: | Sodium Azide |
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