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| Catalog Code: | NB120-17786 |
| Product Name: | Cdk1 Antibody |
| Product Description: | Mouse Monoclonal anti-Cdk1 (SPM234) |
| Clone: | SPM234 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant fragment (Xenopus laevis). C-Terminal 2/3rds of Xenopus cdc2 expressed in E.coli |
| Epitope: | aa220-227 |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Xenopus laevis, Chicken, Guinea pig and Woodchuck.Not yet tested in other species. |
| Packaging: | 7 ml IgG purified Mouse IgG. |
| Uses: | IHC-P: Ready-to-use for 30 min at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | n/a |
| Host Name: | Mouse |
| Buffer: | 10mM PBS, pH 7.4 with BSA |
| Application Summary: | immunohistochemistry-P |
| Species Summary: | Ch, Hu, Mu, Rt, Gp, Xp, |
| Alternate Names: | anti-Cdc 2 antibody, anti-CDK 1 antibody, anti-CDK1 antibody, anti-Cell division control protein 2 antibody, anti-Cell division control protein 2 homolog antibody, anti-Cell Division Cycle 2 antibody, anti-cell division cycle 2 G1 to S and G2 to M antibody, anti-Cell Divsion Cycle 2 Protein antibody, anti-Cyclin Dependent Kinase 1 antibody, anti-DKFZp686L20222 antibody, anti-MGC111195 antibody, anti-p34 antibody, anti-p34 Cdk1 antibody, anti-p34 protein kinase antibody |
| Package Size: | 7 ml |
| Preservative: | Sodium Azide |
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