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| Catalog Code: | NB120-17155 |
| Product Name: | Cytokeratin Antibody |
| Product Description: | Mouse Monoclonal anti-Cytokeratin (Lu-5) |
| Clone: | Lu-5 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Lung cancer cell line. |
| Specificity: | This antibody reacts with cytokeratins of acidic as well as basic subfamilies, i.e. 1,2,3,4,5,6,7,8,10,12,13,14,15,16,17,18 and 19. It reacts with keratinized and corneal epidermis, stratified epithelia, hyperproliferative keratinocytes and simple epithelia. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Cow, Chicken, Frog and Snake.Not yet tested in other species. |
| Packaging: | 1 ml IgG purified Mouse IgG. |
| Uses: | IHC-P: 1/50 - 1/75 in an ABC method, we suggest an incubation period of 30 minutes at room temperature. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. There are two types of cytokeratins the acidic type I cytokeratins and the basic or neutral type II cytokeratins. Cytokeratins are usually found in pairs comprising a type I cytokeratin and a type II cytokeratin. Usually the type II cytokeratins are 8kD larger than their type I counterparts. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry-P |
| Species Summary: | Bv, Ch, Hu, Rt, |
| Package Size: | 1 ml |
| Preservative: | Sodium Azide |
| General References: | General / background references:von Overbeck J et al. Immunohistochemical characterization of an anti-epithelial monoclonal antibody (mAB lu-5). Virchows Arch A Pathol Anat Histopathol 407:1-12 (1985). |
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