| request information: |
|
| Catalog Code: | NB120-17153 |
| Product Name: | Cytokeratin Antibody |
| Product Description: | Mouse Monoclonal anti-Cytokeratin (5D3 + LP34) |
| Clone: | 5D3 + LP34 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Cytokeratins from human breast carcinoma cell line MCF-7 and detergent insoluble fraction of psoriatic epidermis. |
| Specificity: | This antibody stains cytokeratin 5,6,8 and 18 and has been designed for a broad spectrum screening of cytokeratins. It stains carcinomas of simple and squamous origin. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.5 ml purified Mouse IgG. |
| Uses: | IHC-P: 1/15 - 1/50 (an incubation period of 30 minutes at room temperature is recommended). Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Cytokeratins, a group comprising at least 29 different proteins, are characteristic of epithelial and trichocytic cells. Cytokeratins 1, 4, 5, 6, and 8 are members of the type II neutral to basic subfamily. Monoclonal anti cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification. Monoclonal Anti Pan Cytokeratin (mixture) is a broadly reactive reagent, which recognizes epitopes present in most human epithelial tissues. It facilitates typing of normal, metaplastic and neoplastic cells. Synergy between the various components results in staining amplification. This enables identification of cells, which would otherwise be stained only marginally. The mixture may aid in the discrimination of carcinomas and nonepithelial tumors such as sarcomas, lymphomas and neural tumors. It is also useful in detecting micrometastases in lymph nodes, bone marrow and other tissues and for determining the origin of poorly differentiated tumors. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG |
| Host Name: | Mouse |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry-P |
| Species Summary: | Hu, |
| Package Size: | 0.5 ml |
| Preservative: | none |
| Notes Main: | This antibody is a cocktail of two different clones; 5D3 (IgG2a) and LP53 (IgG1). |
| General References: | General / background references:Angus B et al. Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1. J Pathol 155:71-5 (1988). Gatter KC et al. Use of monoclonal antibodies for the histopathological diagnosis of human malignancy. J Clin Pathol 35:1253-67 (1982). |
order or
more information: | company product webpage |