| CatalogCode: | NB120-17146 |
| ProductName: | Actin Antibody |
| Product Description: | Mouse Monoclonal anti-Actin (1A4+5C5) |
| Clone: | 1A4+5C5 |
| Clonality: | Monoclonal |
| Immunogen: | BALB/C mice were injected with N-terminal decapeptide of alpha-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5. |
| CrossReactivity: | Cross-reacts with Human, Rat, Rabbit, Cow, Guinea pig, Sheep and Snake.Not yet tested in other species. |
| Packaging: | 0.5 ml |
| Uses: | IHC-P: 1/25 - 1/50. An incubation period of 30 minutes at room temperature usingf an ABC method is recommended.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | Skeletal muscle |
| Background: | Useful in identifying tumors arising from muscle cells, i.e. leiomyosarcoma as well as rhabdomyosarcoma. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG2a kappa |
| Host_Name: | Mouse |
| Buffer: | Preservative: Sodium Azide |
| ListPrice: | 330 |
| AppSummary: | IHC-P |
| SpeciesSummary: | Hu, Rt, Rb, Bv, GP, Sh, Rp |
| ALTnames: | anti-ActG antibody, anti-ActG1 antibody, anti-Actin antibody, anti-Gamma1 antibody, anti-DFNA20 antibody |
| ProteinTarget: | Actin |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Babai F et al. Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms. Virchows Arch B Cell Pathol Incl Mol Pathol 55:263-77 (1988). Skalli O et al. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas. Am J Pathol 130:515-31 (1988). Schürch W et al. Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors. Am J Pathol 128:91-103 (1987). |
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