| CatalogCode: | | NB120-17130 |
| ProductName: | | Cytokeratin 5 Antibody |
| Product Description: | | Mouse Monoclonal anti-Cytokeratin 5 (XM26) |
| Clone: | | XM26 |
| Clonality: | | Monoclonal |
| Immunogen: | | Prokaryotic recombinant protein corresponding to 103 amino acid portion of the C-terminal region of the cytokeratin 5 molecule (Human). |
| CrossReactivity: | | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | | 0.5 ml Tissue culture supernatant Mouse ascites. |
| Uses: | | IHC-P: An incubation period of 30 minutes at room temperature is recommended. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Localization: | | Cytoplasmic |
| Control: | | Skin |
| Background: | | Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Tissue culture supernatant |
| Isotype: | | IgG1 kappa |
| Host_Name: | | Mouse |
| Buffer: | | Constituents: Tissue culture supernatant |
| ListPrice: | | 295 |
| AppSummary: | | IHC-P |
| SpeciesSummary: | | Hu |
| ALTnames: | | anti-58 kDa cytokeratin antibody, anti-CK 5 antibody, anti-CK5 antibody, anti-Cytokeratin5 antibody, anti-DDD antibody, anti-EBS 2 antibody, anti-EBS2 antibody, anti-K5 antibody, anti-Keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types) antibody, anti-Keratin 5 antibody, anti-Keratin Type II Cytoskeletal 5 antibody, anti-Keratin5 antibody, anti-KRT 5 antibody, anti-KRT 5A antibody, anti-KRT5 antibody, anti-KRT5A antibody |
| ProteinTarget: | | Cytokeratin 5 |
| PackageSize: | | 0.5 ml |
| GeneralRef: | | General / background references:Whittock NV et al. Genomic organization and amplification of the human epidermal type II keratin genes K1 and K5. Biochem Biophys Res Commun 274:149-52 (2000). Irvine AD et al. A mutation in the V1 domain of keratin 5 causes epidermolysis bullosa simplex with mottled pigmentation. J Invest Dermatol 108:809-10 (1997). |
| more information: | | company product webpage |