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| Catalog Code: | NB120-17127 |
| Product Name: | ALK Antibody |
| Product Description: | Mouse Monoclonal anti-ALK (5A4) |
| Clone: | 5A4 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus, corresponding to amino acids 419-520 of Human ALK. |
| Cross Reactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (85% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.5 ml Mouse Tissue culture supernatant |
| Uses: | IHC-P: 1/25 - 1/70. An incubation period of 60 minutes at room temperature is recommendedc using an ABC method. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane |
| Background: | Anaplastic lymphoma kinase (ALK) was originally discovered as a NPM (Nucleophosmin)-ALK fusion protein. ALK and LTK (leukocyte tyrosine kinase) are receptor tyrosine kinases similar to the insulin receptor subfamily of kinases. LTK is found in murine B Lymphocyte precursors and in forebrain neurons. ALK is usually found in the nervous system. A truncated form of ALK occurs in many non Hodgkin's lymphomas. The ALK gene is on chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM ALK) resulting from t(2;5) translocation is a protein of 80kDa with the N terminal portion of NPM linked to the complete intracellular portion of ALK. Karpas 299 and SUDHL1 lymphoid cell lines strongly express NPM ALK. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Application Summary: | Immunohistochemistry-Paraffin |
| Species Summary: | Hu, |
| Alternate Names: | anti-ALK tyrosine kinase receptor antibody, anti-ALK Tyrosine Kinase Receptor Precursor antibody, anti-Anaplastic lymphoma kinase Ki 1 antibody, anti-Anaplastic lymphoma kinase Ki1 antibody, anti-Anaplastic Lymphoma Kinase p80 antibody, anti-CD 246 antibody, anti-CD246 antibody, anti-CD246 Antigen antibody, anti-Ki 1 antibody, anti-Ki1 antibody, anti-TFG/ALK antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% Sodium Azide. |
| General References: | General / background references:Pittaluga S et al. The monoclonal antibody ALK1 identifies a distinct morphological subtype of anaplastic large cell lymphoma associated with 2p23/ALK rearrangements. Am J Pathol 151:343-51 (1997). Pulford K et al. Detection of anaplastic lymphoma kinase (ALK) and nucleolar protein nucleophosmin (NPM)-ALK proteins in normal and neoplastic cells with the monoclonal antibody ALK1. Blood 89:1394-404 (1997). Downing JR et al. Molecular detection of the (2;5) translocation of non-Hodgkin's lymphoma by reverse transcriptase-polymerase chain reaction. Blood 85:3416-22 (1995). |
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