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| Catalog Code: | NB120-17087 |
| Product Name: | Estrogen Receptor Antibody |
| Product Description: | Mouse Monoclonal anti-Estrogen Receptor (1D5) |
| Clone: | 1D5 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. However, on cryostat sections a positive staining of estrogen receptor in the nucleus as well as cytoplasm can be seen. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.25 ml IgG purified Mouse IgG. |
| Uses: | IHC-P: 1/25 - 1/50. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostainingWB: 1/50 - 1/100. Predicted molecular weight: 66 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | This antibody is specific to estrogen receptor, which is associated with superior prognosis and a better response to endocrine therapy. The nuclei of the estrogen receptor positive cells stain very strongly with this antibody, without any staining in the cytoplasm. However, on cryostat sections a positive staining of estrogen receptor in the nucleus as well as cytoplasm can be seen. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 kappa |
| Host Name: | Mouse |
| Application Summary: | Immunohistochemistry-Paraffin, Western Blot, |
| Species Summary: | Hu, |
| Alternate Names: | anti-DKFZp686N23123 antibody, anti-ER alpha antibody, anti-ER antibody, anti-Era antibody, anti-ESR 1 antibody, anti-ESR antibody, anti-ESR1 antibody, anti-ESRA antibody, anti-Estradiol receptor antibody, anti-Estrogen receptor 1 antibody, anti-Estrogen receptor antibody, anti-Major ORF antibody, anti-NR3A1 antibody |
| Package Size: | 0.25 ml |
| Preservative: | Sodium Azide |
| General References: | General / background references:Bur ME et al. Estrogen receptor immunohistochemistry in carcinoma in situ of the breast. Cancer 69:1174-81 (1992). Graham DM et al. Detection of estrogen receptor in paraffin-embedded sections of breast carcinoma by immunohistochemistry and in situ hybridization. Am J Surg Pathol 15:475-85 (1991). Pertschuk LP et al. Immunocytochemical estrogen and progestin receptor assays in breast cancer with monoclonal antibodies. Histopathologic, demographic, and biochemical correlations and relationship to endocrine response and survival. Cancer 66:1663-70 (1990). Kumar V et al. Functional domains of the human estrogen receptor. Cell 51:941-51 (1987). |
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more information: | company product webpage |