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| Catalog Code: | NB120-17070 |
| Product Name: | Cytokeratin 18 Antibody |
| Product Description: | Mouse Monoclonal anti-Cytokeratin 18 (CY-90) |
| Clone: | CY-90 |
| Clonality: | Monoclonal |
| ListPrice: | 285 |
| Immunogen: | Epidermal carcinoma (A431) and MCF-7 human breast cancer cells (Human). |
| Specificity: | In normal tissues, ab17070 reacts with a large number of epithelial cell types, including ductal and glandular epithelia. This antibody does not react with squamous epithelia. Most of the adenocarcinomas, basal cell carcinomas and benign and malignant epithelial lesions stain with this antibody. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 6 ml Mouse ascites. |
| Uses: | IHC-P: This antibody is prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Proteolytic digestion may be required in some paraffin embedded tissue sections.IHC-Fr: This antibody is prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Application Summary: | immunohistochemistry-Fr, immunohistochemistry-P |
| Species Summary: | Hu, |
| Alternate Names: | anti-CK 18 antibody, anti-CYK18 antibody, anti-Cytokeratin endo B antibody, anti-Keratin 18 antibody, anti-Keratin D antibody, anti-Keratin type I cytoskeletal 18 antibody, anti-Kerd antibody, anti-KRT18 antibody |
| Package Size: | 6 ml |
| Preservative: | Sodium Azide |
| General References: | General / background references:Bartek J et al. A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol 164:215-24 (1991). Lauerova L et al. Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7:495-504 (1988). |
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