| CatalogCode: | NB120-17069 |
| ProductName: | Cytokeratin 7 Antibody |
| Product Description: | Mouse Monoclonal anti-Cytokeratin 7 (OV-TL12/30) |
| Clone: | OV-TL12/30 |
| Clonality: | Monoclonal |
| Immunogen: | BALB/C mice were immunized with ovarian carcinoma cell line, OTN11. |
| CrossReactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 6 ml |
| Uses: | IHC-P: Prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. IHC-Fr: Prediluted and ready to use. An incubation period of 30 minutes at room temperature is recommended. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | Skin |
| Background: | Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. It is present in the transitional epithelium of bladder as well as ovarian and lungepithelia, and occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed. However, Cytokeratin 7 is not expressed by epithelial cells of the gastrointestinal tract, colon or prostate. Keratin 7 is often co expressed with keratin 19. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| ListPrice: | 295 |
| AppSummary: | IHC-P, IHC-Fr |
| SpeciesSummary: | Hu |
| ALTnames: | anti-ck7 antibody, anti-K2C7 antibody, anti-Keratin 55k type ii cytoskeletal antibody, anti-Keratin 7 antibody, anti-Keratin simple epithelial type 1 k7 antibody, anti-Keratin type ii cytoskletal 7 antibody, anti-KRT7 antibody, anti-mgc3625 antibody, anti-Sarcolectin antibody, anti-SCL antibody, anti-Type ii mesothelial keratin k7 antibody |
| ProteinTarget: | Cytokeratin 7 prediluted |
| PackageSize: | 6 ml |
| GeneralRef: | General / background references:van Niekerk CC et al. Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues. J Pathol 165:145-52 (1991). Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). Poels LG et al. Characterization of a hormone-producing ovarian carcinoma cell line. Gynecol Oncol 32:203-14 (1989). |
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