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| Catalog Code: | NB120-16728 |
| Product Name: | CD44 Antibody |
| Product Description: | Mouse Monoclonal anti-CD44 (156-3C11) |
| Clone: | 156-3C11 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Gene ID: | 960 (human) |
| Gene Symbol: | GC11P035199 (human) |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Stimulated human leukocytes. |
| Epitope: | Resistant to digestion by trypsin and chymotrypsin.1 |
| Specificity: | This antibody reacts with red cells pretreated with 2-aminoethylisothiouronium bromide (AET).1 Digestion of intact erythrocytes by trypsin treatment leaves a 50-54kDa protein fragment attached to the membrane, while chymotrypsin leaves a somewhat similarly sized 51-56kDa component that are recognized by Ab-4.1 This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA, or CD2.1 Ab-4 enhances proliferation of PBL stimulated with CD2.1 |
| Cross Reactivity: | Cross-reacts with Human, Baboon and Green Monkey. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Mouse ascites. |
| Uses: | IP: (Native verified) (Use Protein A)IHC: (Formalin/paraffin). Staining of formalin-fixed tissues REQUIRES boilingtissue sections in 10mM citrate buffer, pH 6.0. |
| Localization: | Cell Membrane |
| Background: | CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (HCAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG2a |
| Host Name: | Mouse |
| Buffer: | 10mM PBS, pH 7.4, with 2.0% BSA |
| Application Summary: | immunoprecipitation 2 ug/ml, Western Blot 1-2 ug/ml for 2 hrs at RT, Immunohistochemistry-Paraffin 1-2 ug/ml 30 min at RT |
| Species Summary: | Hu, Mk, Bb, |
| Alternate Names: | Anti-CD44 isoform RC antibody; Anti-CD44 molecule antibody; Anti-CDw44 antibody; Anti-Cell surface glycoprotein CD44 antibody; Anti-ECM III antibody; Anti-ECMR III antibody; Anti-ECMRIII antibody; Anti-Epican antibody; Anti-Extracellular Matrix Receptor III antibody; Anti-GP90 Lymphocyte Homing Adhesion Receptor antibody; Anti-HCAM antibody; Anti-HCELL antibody; Anti-Heparan Sulfate Proteoglycan antibody; Anti-Hermes Antigen antibody; Anti-HUTCH I antibody; Anti-HUTCH1 antibody; Anti-Hyaluronate Receptor antibody; Anti-IN antibody; Anti-Indian blood group antibody; Anti-Inlu Related p80 Glycoprotein antibody; Anti-lhr antibody; Anti-Ly 24 antibody; Anti-MC56 antibody; Anti-MDU2 antibody; Anti-MDU3 antibody; Anti-MGC10468 antibody; Anti-MIC4 antibody; Anti-MUTCH I antibody; Anti-MUTCH1 antibody; Anti-PGP 1 antibody; Anti-PGP1 antibody; Anti-Phagocytic Glycoprotein 1 antibody; Anti-Phagocytic glycoprotein I antibody; Anti-Transmembrane glycoprotein antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% sodium azide |
| General References: | 1. Chaudhry A; Gobl A; Eriksson B; Skogseid B; Oberg K. Different splice variants of CD44 are expressed in gastrinomas but not in other subtypes of endocrine pancreatic tumors. Cancer Research, 1994, 54(4):981-6. 2. Hughes DE; Salter DM; Simpson R. CD44 expression in human bone: a novel marker of osteocytic differentiation. Journal of Bone and Mineral Research, 1994, 9(1):39-44. 3. Penno MB; August JT; Baylin SB; Mabry M; Linnoila RI; Lee VS; Croteau D; Yang XL; Rosada C. Expression of CD44 in human lung tumors. Cancer Research, 1994, 54(5):1381-7. 4. Abbasi AM; Chester KA; Talbot IC; Macpherson AS; Boxer G; Forbes A; Malcolm AD; Begent RH. CD44 is associated with proliferation in normal and neoplastic human colorectal epithelial cells. European Journal of Cancer, 1993, 29A(14):1995-2002. 5. Aho R; Ekfors T; Haltia M; Kalimo H. Pathogenesis of primary central nervous system lymphoma: invasion of malignant lymphoid cells into and within the brain parenchyme. Acta Neuropathologica, 1993, 86(1):71-6. 6. East JA; Mitchell SD; Hart IR. Expression and function of the CD44 glycoprotein in melanoma cell lines. Melanoma Research, 1993, 3(5):341-6. 7. Fukazawa H; Yoshida K; Ichinohasama R; Sawai T; Hiromatsu Y; Mori K; Kikuchi K; Aizawa Y; Abe K; Wall JR. Expression of the Hermes-1 (CD44) and ICAM-1 (CD54) molecule on the surface of thyroid cells from patients with Graves' disease. Thyroid, 1993, 3(4):285-9. 8. Heider KH; Dammrich J; Skroch-Angel P; Muller-Hermelink HK; Vollmers HP; Herrlich P; Ponta H. Differential expression of CD44 splice variants in intestinal- and diffuse-type human gastric carcinomas and normal gastric mucosa. Cancer Research, 1993, 53(18):4197-203. 9. Joensuu H; Klemi PJ; Toikkanen S; Jalkanen S. Glycoprotein CD44 expression and its association with survival in breast cancer. American Journal of Pathology, 1993, 143(3):867-74. 10. Johnson BA; Haines GK; Harlow LA; Koch AE. Adhesion molecule expression in human synovial tissue. Arthritis and Rheumatism, 1993, 36:137-46. 11. Messadi DV; Bertolami CN. CD44 and hyaluronan expression in human cutaneous scar fibroblasts. American Journal of Pathology, 1993, 142(4):1041-9. 12. Penneys NS. CD44 expression in normal and inflamed skin. Journal of Cutaneous Pathology, 1993, 20(3):250-3. 13. Underhill CB; Nguyen HA; Shizari M; Culty M. CD44 positive macrophages take up hyaluronan during lung development. Developmental Biology, 1993, 155(2):324-36. 14. Wielenga VJ; Heider KH; Offerhaus GJ; Adolf GR; van den Berg FM; Ponta H; Herrlich P; Pals ST. Expression of CD44 variant proteins in human colorectal cancer is related to tumor progression. Cancer Research, 1993, 53(20):4754-6. 15. Foets BJ; van den Oord JJ; Volpes R; Missotten L. In situ immunohistochemical analysis of cell adhesion molecules on human corneal endothelial cells. British Journal of Ophthalmology, 1992,76(4):205-9. 16. Skov BG; Hirsch FR; Bobrow L. Monoclonal antibodies in the detection of bone marrow metastases in small cell lung cancer. British Journal of Cancer, 1992, 65(4):593-6. 17. Vogel H; Butcher EC; Picker LJ. H-CAM expression in the human nervous system: evidence for a role in diverse glial interactions. Journal of Neurocytology, 1992, 21(5):363-73. 18. Wang C; Tammi M; Tammi R. Distribution of hyaluronan and its CD44 receptor in the epithelia of human skin appendages. Histochemistry, 1992, 98(2):105-12. 19. Kuppner MC; Hamou MF; de Tribolet N. Activation and adhesion molecule expression on lymphoid infiltrates in human glioblastomas. Journal of Neuroimmunology, 1990, 29(1-3):229-38. |
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