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| Catalog Code: | NB120-16518 |
| Product Name: | CD133 Antibody |
| Product Description: | Rabbit Polyclonal anti-CD133 |
| Clonality: | Polyclonal |
| ListPrice: | 285 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide corresponding to a C-terminal region of CD133 (within amino acids 750-865). |
| Cross Reactivity: | Reacts with Human and mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | ELISA: 1/1000.WB: 1/1,000 - 1:2,000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).IHC: Citrate buffer antigen retreival required.ICC/IF: Fix with 3% paraformaldehyde for 20 min at RT.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | Prominin-1 (also known as CD133) is expressed on primitive hematopoietic stem and progenitor cells, retinoblastoma, hemangioblasts, and neural stem cells as well as on developing epithelium. The Prominin-1 positive fraction of human bone marrow, cord blood and peripheral blood have been shown to efficiently engraft in xenotransplantation models, and have been shown to contain the majority of the granulocyte/macrophage precursors, NOD/SCID repopulating cells and CD34 + dendritic cell precursors. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | ELISA 1:1000, immunohistochemistry , immunofluorescence 1:100, Western Blot 1:1000-1:2000, Immunocytochemistry 1:100, Immunohistochemistry-Paraffin 1:25 |
| Species Summary: | Hu, Mu |
| Alternate Names: | anti-AC133 antibody, anti-PROML1 antibody, anti-PROM antibody, anti-Prominin 1 antibody, anti-CD133 Antigen antibody, anti-CD133 antibody, anti-CD133 Retinal Degeneration, Autosomal Recessive, Prominin-Related antibody, anti-Prominin-1 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% sodium azide |
| Homology: | Sequence is 87% identical and 100% positive in mouse and rat. |
| General References: | Yin, AH, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 90: 5002-5012 (1997) 2. Yu, Y, et al. AC133-2, a novel isoform of human AC133 stem cell antigen. J. Biol. Chem. 277: 20711-20716 (2002) 3. Singh SK, et al. Identification of human brain tumour initiating cells. Nature. 432: 396-401 (2004) PUBMED:15549107 4. Alessandri G, et al. Isolation and culture of human muscle-derived stem cells able to differentiate into myogenic and neurogenic cell lineages. Lancet 364: 1872-83 (2004) PUBMED:15555667 5. Fauth F, et al. AC133 expression on acute myeloid leukemia blasts: correlation to FAB and to CD34 expression and possible implications for peripheral blood progenitor cell purging in AML. Leuk Res. 25: 191-6 (2001) PUBMED:11226513 |
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