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| Catalog Code: | NB120-15862 |
| Product Name: | TFIIF Antibody |
| Product Description: | Chicken Polyclonal anti-TFIIF |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide, corresponding to amino acids 1-249 of Human TFIIF. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Chicken IgY |
| Uses: | WB: 1/2000. Predicted molecular weight: 75 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | The RNA polymerase II transcription factor TFIIF is a heterodimer with 2 subunits: one, referred to as RAP74, of approximately 70 kD and the other, known as RAP30 or GTF2F2, of 30 kD. TFIIF affects RNA polymerase II activity both during the initiation and elongation stages of RNA transcription. TFIIF appears to assist TFIIB in recruiting RNA polymerase II into a preinitiation complex. It also affects RNA transcription by suppressing transient pausing of the polymerase at locations on the DNA template. TFIIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-ATP dependent helicase GTF2F2 antibody, anti-BTF 4 antibody, anti-BTF4 antibody, anti-General transcription factor IIF polypeptide 1 antibody, anti-General transcription factor IIF polypeptide 1 74 kDa subunit protein antibody, anti-General transcription factor IIF polypeptide 1 74kDa antibody, anti-General transcription factor IIF polypeptide 2 30kDa antibody, anti-General Transcription Factor IIF Polypeptide 2 antibody, anti-GTF2F1 antibody, anti-GTF2F1 protein antibody, anti-GTF2F2 antibody, anti-RAP 30 antibody, anti-RAP 74 antibody, anti-RAP30 antibody, anti-RAP74 antibody, anti-TF2F 1 antibody, anti-TF2F 2 antibody, anti-TF2F1 antibody, anti-TF2F2 antibody, anti-TFIIF alpha antibody, anti-TFIIF beta antibody, anti-Transcription initiation factor IIF alpha subunit antibody, anti-Transcription Initiation Factor IIF beta Subunit antibody, anti-Transcription initiation factor IIF subunit beta antibody, anti-Transcription initiation factor RAP30 antibody, anti-Transcription initiation factor RAP74 antibody |
| Package Size: | 0.05 mg |
| Preservative: | None |
| General References: | General / background references:Sopta M et al. Structure and associated DNA-helicase activity of a general transcription initiation factor that binds to RNA polymerase II. Nature 341:410-4 (1989). |
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