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| Catalog Code: | NB120-15524 |
| Product Name: | STAT3 Antibody |
| Product Description: | Rabbit Polyclonal anti-STAT3 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Recombinant fragment: E SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY, corresponding to amino acids 50-240 of Human STAT3. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Expected to cross-react with Xenopus laevis (85% identity with immunogen), Cow (92% identity with immunogen) and Chicken (91% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 7 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IHC-P: Ready to use for 10 min at room temperature. Staining of formalin fixed tissues requires boiling tissue sections in 10mMcitrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear. |
| Background: | STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5. Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage. STAT1 and STAT2 are induced by IFN alpha and forn 1, a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, BSA |
| Application Summary: | immunohistochemistry-P |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-STAT3 antibody, anti-signal transducer and activator of transcription 3 (acute-phase response factor) antibody, anti-APRF antibody, anti-FLJ20882 antibody, anti-MGC16063antibody, anti-DNA-binding protein APRF antibody, anti-acute-phase response factor antibody, anti-signal transducer and activator of transcription 3 antibody |
| Package Size: | 7 ml |
| Preservative: | Sodium Azide |
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