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| Catalog Code: | NB120-15472 |
| Product Name: | MDM2 Antibody |
| Product Description: | Rabbit Polyclonal anti-MDM2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: SRPSTSSRRRAISE, corresponding to amino acids 154-167 of Human MDM2. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 7 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IHC-P: Use undiluted. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localisation of both proteins to the nucleus. The nucleolar localisation signals in both ARF and MDM2 may be necessary to allow efficient nucleolar localisation of both proteins. |
| Background: | MDM2 is a nuclear phosphoprotein with an apparent molecular mass of 90 kD that forms a complex with the p53 tumor suppressor protein. Human MDM2 was identified as a homologous product of the 'murine double minute 2' gene (mdm2). The MDM2 gene enhances the tumorigenic potential of cells when it is overexpressed and encodes a putative transcription factor. Forming a tight complex with the p53 gene, the MDM2 oncogene can inhibit p53 mediated transactivation, MDM2 also binds to p53 protein. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a key factor in human tumorigenesis. p53 can be subjected to negative regulation by the product of a single cellular protooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity of p53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53. MDM2 p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing 5 additional splice variant transcripts from the full length MDM2 gene. The alternatively spliced transcripts tend to be expressed in tumorigenic tissue, whereas the full length MDM2 transcript is expressed in normal tissue. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, BSA |
| Application Summary: | immunohistochemistry-P |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-MDM2 antibody, anti-Mdm2 antibody, anti-transformed 3T3 cell double minute 2 antibody, anti-p53 binding protein (mouse) antibody, anti-HGNC:6973 antibody, anti-MGC71221 antibody, anti-hdm2 antibody, anti-mouse double minute 2 homolog antibody, anti-mouse double minute 2 antibody, anti-human homolog of antibody, anti-p53-binding protein antibody, anti-p53-binding protein MDM2 antibody, anti-ubiquitin-protein ligase E3 Mdm2 antibody |
| Package Size: | 7 ml |
| Preservative: | Sodium Azide |
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more information: | company product webpage |