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| Catalog Code: | NB120-15326 |
| Product Name: | iNOS Antibody |
| Product Description: | Rabbit Polyclonal anti-iNOS |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide (Mouse) (C terminal). |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 7 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2). |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | prediluted antibody |
| Application Summary: | Immunohistochemistry-Paraffin |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-HEP NOS antibody, anti-Hepatocyte NOS antibody, anti-HEPNOS antibody, anti-Inducible nitric oxide synthase antibody, anti-Inducible NO synthase antibody, anti-Inducible NOS antibody, anti-INOS antibody, anti-Inosl antibody, anti-MAC NOS antibody, anti-Macrophage NOS antibody, anti-Nitric oxide synthase 2 inducible macrophage antibody, anti-Nitric oxide synthase 2A (inducible hepatocytes) antibody, anti-Nitric oxide synthase inducible antibody, anti-NOS 2 antibody, anti-NOS 2A antibody, anti-NOS antibody, anti-Nos II antibody, anti-NOS type II antibody, anti-Nos2 antibody, anti-NOS2A antibody |
| Package Size: | 7 ml |
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more information: | company product webpage |
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