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| Catalog Code: | NB120-15195 |
| Product Name: | Cyclin B1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cyclin B1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide (Human) (C terminal). |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 7 ml Immunogen affinity purified Rabbit IgG |
| Uses: | IHC-P: Ready diluted. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Inactive cyclin B cytoplasmic. |
| Background: | In mammals, cyclin B associates with inactive p34 cdc2 which facilitates phosphorylation of p34 cdc2 at amino acids 14 Thr and 15 Tyr. This maintains the inactive state until the end of G2 phase. The inactive cyclin B p34 cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids 14Thr and 15Tyr of p34 cdc2, rendering the complex active at the G2 / M boundary. This mitotic kinase complex remains active until the metaphase / anaphase transition when cyclin B is degraded. This degradation process is ubiquitin dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B p34 cdc2 plays a critical role in G2 to M transition. Two alternative transcripts have been found, a constitutively expressed transcript, and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | BSA, 10mM PBS. pH 7.4 |
| Application Summary: | immunohistochemistry-P |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-CCNB 1 antibody, anti-CCNB antibody, anti-CCNB1 antibody, anti-G2 mitotic specific cyclin B1 antibody |
| Package Size: | 7 ml |
| Preservative: | Sodium Azide |
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more information: | company product webpage |