| CatalogCode: | NB120-15137 |
| ProductName: | ErbB 4 Antibody |
| Product Description: | Rabbit Polyclonal anti-ErbB 4 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal). |
| CrossReactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.5 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IHC-P: Use at a dilution of 1/50 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.IP: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 146.8 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein |
| Control: | Breast carcinoma or skeletal muscle. |
| Background: | Epidermal Growth Factor (EGF) affects cell growth through its interaction with a 170 kDa cell surface glycoprotein, known as the EGF receptor. Binding of EGF or TGF alpha to the receptor activates its associated kinase. Other members of the EGF receptor family include Neu (ErbB 2, HER2), ErbB 3 (HER3) and ErbB 4 (HER4). The cytoplasmic regions of EGFR and Neu are quite similar, but ErbB 3 and ErbB 4 more closely resemble each other than EGFR or Neu. ErbB 4 is the fourth member of class I receptor kinase family. ErbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47D, MDA MB 453, BT 474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF7, MDAMB 330 and MDA MB 361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA MB 231, MDA MB 157, MDA MB 468, and SKBR 3. Down-regulation of ErbB 4 in estrogen receptor-positive cancer cells inhibits proliferation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | BSA, 10mM PBS. pH 7.4 |
| ListPrice: | 285 |
| AppSummary: | IHC-P, IP, WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-Avian erythroblastic leukemia viral oncogene homolog 4 antibody, anti-Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody, anti-ErbB4 antibody, anti-HER 4 antibody, anti-HER4 antibody, anti-MGC138404 antibody, anti-P180erbB4 antibody, anti-Receptor protein tyrosine kinase erbB 4 precursor antibody, anti-Receptor tyrosine protein kinase erbB 4 antibody, anti-Tyrosine kinase type cell surface receptor HER4 antibody, anti-v erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody, anti-v erb a erythroblastic leukemia viral oncogene homolog 4 antibody, anti-Verba avian erythroblastic leukemia viral oncogene homolog like 4 antibody, anti-Verba erythroblastic leukemia viral oncogene homolog 4 antibody, anti-VERBB2 antibody |
| ProteinTarget: | ErbB 4 |
| PackageSize: | 0.5 ml |
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