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| Catalog Code: | NB120-14821 |
| Product Name: | alpha A Crystallin Antibody |
| Product Description: | Mouse Monoclonal anti-alpha A Crystallin (c9F2) |
| Clone: | c9F2 |
| Clonality: | Monoclonal |
| ListPrice: | 335 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Recombinant full length protein (Human). |
| Cross Reactivity: | Cross-reacts with Human and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml protein G purified Mouse ascites. |
| Uses: | ELISA, WB: 1/1000 - 1/2000. NB 120-14821 detects alpha A Crystallin from rat eye extracts at a dilution of 1/1000. Proteins are visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAB detection system. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Background: | Alpha crystallins are major proteins of the ocular lens and are thought to play a role in maintaining lens transparency. They are composed of two gene products alpha A and alpha B, for acidic and basic, respectively. Alpha-crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP) family. They act as molecular chaperones and hold unfolded or misfolded proteins in large, water-soluble low molecular weight aggregates. These heterogeneous aggregates consist of 30-40 subunits of alpha crystallins, and the alpha A and alpha B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and the participation in the intracellular architecture. |
| Storage: | Store short term at 4C. Aliquot and store at -20C long term. |
| Purity: | protein G purified |
| Isotype: | IgG1 kappa |
| Host Name: | Mouse |
| Buffer: | PBS. pH 7.4 with 10% glycerol |
| Application Summary: | ELISA Use at an assay dependent dilution, Western Blot 1/1000 - 1/2000 |
| Species Summary: | Hu, Rt, |
| Alternate Names: | anti-Heat shock protein beta 4 antibody, anti-Acry-1 antibody, anti-CRYA 1 antibody, anti-CRYA1 antibody, anti-CRYAA antibody, anti-Crystallin Alpha 1 antibody, anti-HSPB4 antibody, anti-Zonular Central Nuclear Cataract antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.1% Sodium Azide |
| General References: | 1. Andley, U. P. et al. (1996) Cloning, expression, and chaperone-like activity of human alphaA-crystallin. J. Biol. Chem. 271: 31973-31980. |
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more information: | company product webpage |