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| Catalog Code: | NB120-14311 |
| Product Name: | MMP2 Antibody |
| Product Description: | Chicken Polyclonal anti-MMP2 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: CPKESCNLFVL KDTLKKMQKF FGLPQTGDLD QNTIETMRKP RCGNPDVANY NFFPRKPKWD KNQITYRIIG YTPDLDPETV DDAFARAFQV WSDVTPLRFS RIHDGEADIM INFGRWEHGD, corresponding to amino acids 60-180 of MMP2. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat. Expected to cross-react with Cow (97% identity with immunogen), Chicken (92% identity with immunogen), Dog (97% identity with immunogen), Pig (96% identity with immunogen) and Rabbit (96% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Chicken IgY |
| Uses: | IHC: Use at a dilution of 1/200. WB: Use at a dilution of 1/2000. Predicted molecular weight: 72 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Secreted |
| Background: | The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zinc endopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. MMP2, also known as Gelatinase A, is a type IV collagenase that specifically cleaves type IV collagen, the major structural component of basement membranes. The metastatic potential of tumor cells has been found to correlate with the activity of this enzyme. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | immunohistochemistry, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-72 kDa gelatinase antibody, anti-72kD type IV collagenase antibody, anti-CLG 4 antibody, anti-CLG 4A antibody, anti-CLG4 antibody, anti-CLG4A antibody, anti-Collagenase Type 4 alpha antibody, anti-Gelatinase A antibody, anti-Gelatinase alpha antibody, anti-Gelatinase neutrophil antibody, anti-Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase antibody, anti-Matrix Metalloproteinase 2 antibody, anti-MMP 2 antibody, anti-MMP II antibody, anti-MONA antibody, anti-TBE 1 antibody |
| Package Size: | 0.05 mg |
| Preservative: | None |
| Notes Main: | During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap. |
| General References: | General / background references:Morgunova E et al. Structure of human pro-matrix metalloproteinase-2: activation mechanism revealed. Science 284:1667-70 (1999). |
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