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| Catalog Code: | NB120-14290 |
| Product Name: | Dnmt1 Antibody |
| Product Description: | Chicken Polyclonal anti-Dnmt1 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.91 mg/ml |
| Immunogen: | Synthetic peptide, corresponding to amino acids 177-550 of Human Dnmt1. |
| Cross Reactivity: | Cross-reacts with Human and Bovine. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Chicken IgY |
| Uses: | ICC: Use at a dilution of 1/200. Western Blot: Use at a dilution of 1/1000 - 1/2000. Predicted molecular weight: 194 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt 1, Dnmt 2 and Dnmt 3. Dnmt 1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt 2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt 3a and Dnmt 3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F 1, and HDAC 1. Dnmt 1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | Immunocytochemistry, Western Blot, |
| Species Summary: | Bv, Hu, |
| Alternate Names: | anti-DNMT-1 antibody, anti-DNMT 1 antibody, anti-DNMT antibody, anti-DNA 9cytosine-5-)-methylatransferase 1 antibody, anti-MCMT antibody, anti-DNA MTase Mmu1 antibody, anti-DNA methyltransferase M.HsaI antibody |
| Package Size: | 0.05 mg |
| Preservative: | none |
| Notes Main: | During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 uL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap. |
| General References: | General / background references:Rountree MR et al. DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci. Nat Genet 25:269-77 (2000). |
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