| CatalogCode: | | NB120-14243 |
| ProductName: | | p27 KIP 1 Antibody |
| Product Description: | | Chicken Polyclonal anti-p27 KIP 1 |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide, corresponding to amino acids 1-198 of p27 KIP 1. |
| CrossReactivity: | | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | | 0.05 mg Immunogen affinity purified Chicken IgY. |
| Uses: | | ICC: Use at a dilution of 1/200. IHC-Fr: Use at a dilution of 1/200. WB: Use at a dilution of 1/2000. Predicted molecular weight: 22 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Nuclear |
| Background: | | Cell cycle progression is regulated by cyclins and their cognate Cdks. p27 KIP 1 is a cell cycle regulatory mitotic inhibitor of cdk activity. p27 KIP 1 is a candidate tumor suppressor gene, and has been proposed to function as a possible mediator of TGF beta induced G1 arrest. p27 KIP 1 is up regulated in response to antimitogenic stimuli. The increased protein expression of p27 results in cellular arrest by binding to cyclin/Cdk complexes such as cyclin D1/Cdk4. p27 Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27 Kip 1; while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm. |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgY |
| Host_Name: | | Chicken |
| Buffer: | | PBS |
| ListPrice: | | 285 |
| AppSummary: | | ICC, IHC-Fr, WB |
| ALTnames: | | anti-CDKN 1B antibody, anti-CDKN 4 antibody, anti-CDKN1B antibody, anti-CDKN4 antibody, anti-Cyclin Dependent Kinase Inhibitor 1B antibody, anti-Cyclin dependent kinase inhibitor p27 antibody, anti-KIP 1 antibody, anti-KIP1 antibody, anti-MEN1B antibody, anti-p27 Kip1 antibody, anti-P27KIP1 antibody |
| ProteinTarget: | | p27 KIP 1 |
| PackageSize: | | 0.05 mg |
| GeneralRef: | | General / background references:Polyak K et al. Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals. Cell 78:59-66 (1994). |
| more information: | | company product webpage |