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| Catalog Code: | NB120-14227 |
| Product Name: | AP3B2 Antibody |
| Product Description: | Chicken Polyclonal anti-AP3B2 |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1.3 mg/ml |
| Immunogen: | Synthetic peptide: SLLEENAEK AFYGSEEDEA KGAGSEETAA AAAPSRKPYV MDPDHRLLLR NTKPLLQSRSA AVVMAVAQLYFH LAPKAEVGVIAK ALVRLLRS , corresponding to amino acids 259-350 of Human AP3B2. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Chicken IgY. |
| Uses: | ICC: Use at a dilution of 1/200. IHC: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Golgi Apparatus |
| Background: | Adaptor protein complex AP-3 beta-2 (AP3B2) is a subunit of non-clathrin- and clathrin-associated adaptor protein complex 3 that plays a role in protein sorting in the late-Golgi/trans-Golgi network and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. AP-3 appears to be involved in the sorting of a subset of transmembrane proteins targeted to lysosomes and lysosome-related organelles. Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta/AP3D1 and beta3A/AP3B2 or beta3B/AP3B1), a medium adaptin (mu3A/AP3M1 or mu3B/AP3M2) and a small adaptin (sigma3A/AP3S1 or sigma3B/AP3S2). AP3 is a component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. AP3B2 expression is specific to nervous system (nerve terminal, cell body), and is associated with nerve-terminal vesicles. Expression observed in Purkinje cells, cortical neurons, neuroectodermal tumors and graded in cerebral cortex (deeper layers exhibit stronger expression). AP3B2 is expressed from early in development through adulthood. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | Immunocytochemistry, immunohistochemistry, |
| Species Summary: | Hu, |
| Alternate Names: | anti-Adaptor related protein complex 3 beta 2 subunit antibody, anti-Adaptor-related protein complex 3 beta 2 subunit antibody, anti-AP 3 complex subunit beta 2 antibody, anti-beta NAP antibody, anti-Beta3B adaptin antibody, anti-Clathrin assembly protein complex 3 beta 2 large chain antibody, anti-NAPTB antibody, anti-Neuron specific vesicle coat protein and cerebellar degeneration antigen antibody, anti-Neuron-specific vesicle coat protein and cerebellar degeneration antigen antibody, anti-Neuronal adaptin like protein beta subunit antibody, anti-Neuronal adaptin-like protein beta-subunit antibody |
| Package Size: | 0.05 mg |
| Preservative: | none |
| General References: | General / background references:Darnell RB et al. Antiserum from a patient with cerebellar degeneration identifies a novel protein in Purkinje cells, cortical neurons, and neuroectodermal tumors. J Neurosci 11:1224-30 (1991). |
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