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| Catalog Code: | NB120-14059 |
| Product Name: | NFkB Antibody |
| Product Description: | Chicken Polyclonal anti-NFkB |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Recombinant fragment, corresponding to amino acids 432-499 of Human NFkB. |
| Specificity: | The immunogen that was used to create this antibody has 7 ANK repeats, 1 death domain, and 1 RHD (Rel-like) domain. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat. |
| Packaging: | 0.05 mg IgG purified Chicken antisera. |
| Uses: | WB: Use at a dilution of 1:2000. Predicted molecular weight: 105 kDa. |
| Localization: | Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). |
| Background: | NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | Western Blot 1:2000 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-DNA binding factor KBF1 EBP1 antibody, Anti-DKFZp686C01211 antibody, Anti-DNA binding factor KBF1 antibody, Anti-EBP 1 antibody, Anti-EBP1 antibody, Anti-KBF1 antibody, Anti-MGC54151 antibody, Anti-NF kappa B antibody, Anti-NFKB 1 antibody, Anti-NFKB p105 antibody, Anti-NFKB p50 antibody, Anti-NFKB1 antibody, Anti-Nuclear factor kappa B DNA binding subunit antibody, Anti-Nuclear factor NF kappa B p105 subunit antibody, Anti-Nuclear factor NF kappa B p50 subunit antibody, Anti-Nuclear factor NF-kappa-B p65 subunit antibody, Anti-Nuclear factor of kappa light polypeptide gene enhancer in B cells 1 antibody, Anti-Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 p65 (avian) antibody, Anti-V-rel avian reticuloendotheliosis viral oncogene homolog A antibody |
| Package Size: | 0.05 mg |
| Preservative: | No preservatives |
| General References: | 1. Hou,S., Guan,H. and Ricciardi,R.P., et al. Phosphorylation of serine 337 of NF-kappaB p50 is critical for DNA binding. 2. Majumdar,S. and Aggarwal,B.B., et al. Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types 3. Cogswell,P.C., Kashatus,D.F., Keifer,J.A., et al. NF-kappa B and I kappa B alpha are found in the mitochondria. Evidence for regulation of mitochondrial gene expression by NF-kappa B. 4. Ladner,K.J., Caligiuri,M.A. and Guttridge,D.C., et al. Tumor necrosis factor-regulated biphasic activation of NF-kappa B is required for cytokine-induced loss of skeletal muscle gene products. 5. Medina,E., Anders,D. and Chhatwal,G.S., et al. Induction of NF-kappaB nuclear translocation in human respiratory epithelial cells by group A streptococci. 6. Kieran M., Blank V., Logeat F., et al. The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product. 7. Bours V., Villalobos J., Burd P.R., et al. Cloning of a mitogen-inducible gene encoding a kappa B DNA-binding protein with homology to the rel oncogene and to cell-cycle motifs. 8. Meyer R., Hatada E.N., Hohmann H.-P., et al. Cloning of the DNA-binding subunit of human nuclear factor kappa B: the level of its mRNA is strongly regulated by phorbol ester or tumor necrosis factor alpha. 9. Heron E., Deloukas P., van Loon A.P.G.M. The complete exon-intron structure of the 156-kb human gene NFKB1, which encodes the p105 and p50 proteins of transcription factors NF-kappa B and I kappa B-gamma: implications for NF-kappa B-mediated signal transduction. 10. Chang H.-M., Tsai S.-F. Genome sequencing of the chromosome 4q region implicated in human hepatocellular carcinoma pathogenesis. |
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