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| Catalog Code: | NB120-14047 |
| Product Name: | Cytokeratin 18 Antibody |
| Product Description: | Chicken Polyclonal anti-Cytokeratin 18 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Recombinant fragment, corresponding to amino acids 386-430 of Human Cytokeratin 18. |
| Specificity: | This antibody, Keratin, type I cytoskeletal 18 reacts with human, mouse, rat. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Chicken antisera. |
| Uses: | WB: Use at a dilution of 1/500. Predicted molecular weight: 54 kDa. Not tested in other applications. |
| Background: | Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non keratinizing cells (ie. mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification. Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies that recognize only Cytokeratins 8 and 18. |
| Storage: | 4C for short term, -20C for long term. Avoid frequent freeze and thaw. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | Western Blot 1:500 |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-CK 18 antibody, anti-CYK18 antibody, anti-Cytokeratin endo B antibody, anti-Keratin 18 antibody, anti-Keratin D antibody, anti-Keratin type I cytoskeletal 18 antibody, anti-Kerd antibody, anti-KRT18 antibody |
| Package Size: | 0.05 mg |
| Preservative: | No Preservative |
| Notes Main: | Some researchers have been able to get this antibody to work for IF/ICC. ICC: Use at a dilution of 1/200. |
| General References: | 1. Oshima R.G., Millan J.L., Cecena G. Comparison of mouse and human keratin 18: a component of intermediate filaments expressed prior to implantation. 2. Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. 3. Leube R.E., Bosch F.X., Romano V., Zimbelmann R., Hofler H., Franke W.W. Cytokeratin expression in simple epithelia. III. Detection of mRNAs encoding human cytokeratins nos. 8 and 18 in normal and tumor cells by hybridization with cDNA sequences in vitro and in situ. 4. Romano V., Hatzfeld M., Magin T.M., Zimbelmann R., Franke W.W., Maier G., Ponstingl H. Cytokeratin expression in simple epithelia. I. Identification of mRNA coding for human cytokeratin no. 18 by a cDNA clone. 5. Kulesh D.A., Oshima R.G. Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. 6. Ji H., Reid G.E., Moritz R.L., Eddes J.S., Burgess A.W., Simpson R.J. A two-dimensional gel database of human colon carcinoma proteins. 7. Ku N.-O., Omary M.B. Identification and mutational analysis of the glycosylation sites of human keratin 18. 8. Shi J., Sugrue S.P. Dissection of protein linkage between keratins and pinin, a protein with dual location at desmosome-intermediate filament complex and in the nucleus. 9. Ku N.-O., Wright T.L., Terrault N.A., Gish R., Omary M.B. Mutation of human keratin 18 in association with cryptogenic cirrhosis. 10. Ku N.-O., Darling J.M., Krams S.M., Esquivel C.O., Keeffe E.B., Sibley R.K., Lee Y.M., Wright T.L., Omary M.B. Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies. |
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