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| Catalog Code: | NB120-14014 |
| Product Name: | Dnmt3b Antibody |
| Product Description: | Chicken Polyclonal anti-Dnmt3b |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Recombinant fragment, corresponding to amino acids 271-446 of Human Dnmt3b. |
| Cross Reactivity: | Cross-reacts with Human and Mouse.Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Chicken IgY |
| Uses: | ICC: Use at a dilution of 1/200. Western Blot: Use at a dilution of 1/500. Predicted molecular weight: 96 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome. |
| Storage: | 4 degree C for short term (weeks) and -20 degree C for long term. Avoid frequent freeze and thaw. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgY |
| Host Name: | Chicken |
| Buffer: | PBS |
| Application Summary: | Immunocytochemistry, Western Blot, |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-Cytosine 5methyltransferase 3B antibody, anti-DNA (cytosine 5) methyltransferase 3 beta antibody, anti-DNA antibody, anti-DNA methyltransferase HsaIIIB antibody, anti-DNA MTase HsaIIIB antibody, anti-Dnmt3b antibody, anti-EC 2.1.1.37 antibody, anti-ICF antibody, anti-M.HsaIIIB antibody |
| Package Size: | 0.05 mg |
| Preservative: | none |
| General References: | General / background references:Rhee I et al. DNMT1 and DNMT3b cooperate to silence genes in human cancer cells. Nature 416:552-6 (2002). |
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more information: | company product webpage |