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| Catalog Code: | NB120-13592 |
| Product Name: | CAD Antibody |
| Product Description: | Mouse Monoclonal anti-CAD (36A349) |
| Clone: | 36A349 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (88% identity with immunogen) and Rat (88% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg purified Mouse IgG. |
| Uses: | WB: Use at a concentration of 2 ug/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 41 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear. |
| Background: | Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Application Summary: | Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-Caspase activated deoxyribonuclease antibody, anti-Caspase activated DNase antibody, anti-Caspase activated nuclease antibody, anti-CPAN antibody, anti-DFF2 antibody, anti-DFF40 antibody, anti-DFFB antibody, anti-DNA fragmentation factor 40 kD beta subunit antibody, DNA fragmentation factor 40kDa beta polypeptide antibody |
| Package Size: | 0.1 mg |
| Preservative: | Sodium Azide |
| General References: | General / background references:Enari M et al. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 391:43-50 (1998). Sakahira H et al. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 391:96-9 (1998). Liu X et al. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci U S A 95:8461-6 (1998). Halenbeck R et al. CPAN, a human nuclease regulated by the caspase-sensitive inhibitor DFF45. Curr Biol 8:537-40 (1998). |
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