| CatalogCode: | NB120-13545 |
| ProductName: | CD40 Antibody |
| Product Description: | Rabbit Polyclonal anti-CD40 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: CQPVTQEDGKESRISVQERQ conjugated to KLH, corresponding to amino acids 258-277 of Human CD40. |
| Specificity: | The antibody specificity is confirmed by specific peptide inhibition in immunoblotting studies. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Monkey and Pig. Expected to cross-react with Dog (100% identity with immunogen) and Chicken (88% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg Whole Rabbit antisera. |
| Uses: | WB: Use at a concentration of 2 - 4 ug/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein (isoforms I, III, IV and V); secreted (isoform II). |
| Background: | CD40 (48 to 50 kDa) is a transmembrane glycoprotein mainly expressed on the surface of B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily, which includes the low affinity nerve growth factor (NGF) receptor and CD95/Fas. CD40 is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Following interaction with CD40 ligand, CD40 mediates a number of major immunoregulatory functions, central to the control of thymus dependent humoral immunity and may be critical in the development of cell mediated immune responses. Other biological actions include B cell homotypic adhesion, proliferation, immunoglobulin isotype switch, and secretion. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Ma, Mu, Rt |
| ALTnames: | anti-Bp50 antibody, anti-CDW40 antibody, anti-MGC9013 antibody, anti-TNFRSF5 antibody, anti-p50 antibody |
| ProteinTarget: | CD40 |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Werneburg BG et al. Molecular characterization of CD40 signaling intermediates. J Biol Chem 276:43334-42 (2001). Jana M et al. Ligation of CD40 stimulates the induction of |
| NotesMain: | Storage below -20 degrees C is not recommended. For maximum product recovery centrifuge the product vial before removing cap. Shipped on gel packs. |