| CatalogCode: | | NB120-13538 |
| ProductName: | | Estrogen Receptor Antibody |
| Product Description: | | Mouse Monoclonal anti-Estrogen Receptor (h-151) |
| Clone: | | h-151 |
| Clonality: | | Monoclonal |
| Immunogen: | | Synthetic peptide: RAANLWPSPLMIKR conjugated to KLH, corresponding to amino acids 287-300 of Human Estrogen Receptor. |
| Epitope: | | hinge region |
| Specificity: | | Binding specificity was confirmed in peptide inhibition studies with the intact protein. The antibody does not recognize the native estrogen receptor in the inactive 8S oligomeric state, but reacts efficiently with the activated 4S receptor. In immunoprecipitation studies, this antibody has been shown to only IP the active receptor. |
| CrossReactivity: | | Cross-reacts with Human, Mouse, Rat and Cow. Not yet tested in other species. |
| Packaging: | | 0.1 mg protein G purified Mouse IgG. |
| Uses: | | GSA: Use at an assay dependent dilution. IHC: Use at a dilution of 1/400. IP: Use at a concentration of 15 ug/ml. WB: Use at a concentration of 15 ug/ml. Predicted molecular weight: 71.4 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Nuclear |
| Background: | | Estrogen Receptor alpha is a 65 kDa protein and a member of the steroid family of nuclear receptors. Estrogen Receptor alpha is a ligand-activated transcription factor that, when bound to estrogen, induces a conformational change that allows dimerization and binding to estrogen response element sequences. When bound to DNA, Estrogen Receptor alpha can positively or negatively regulate gene transcription. Like other steroid hormone receptors, Estrogen Receptors are intracellular proteins. Estrogen Receptors play an important role in regulating mammary gland growth and differentiation. Breast tumors that are ER positive may also show a more favorable response to anti-estrogen therapies |
| Storage: | | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | | protein G purified |
| Isotype: | | IgG1 |
| Host_Name: | | Mouse |
| Buffer: | | phosphate buffered saline (PBS) pH 7.2, 50% glycerol |
| ListPrice: | | 285 |
| AppSummary: | | GS, IHC, IP, WB |
| SpeciesSummary: | | Bv, Hu, Ma, Mu, Rt |
| ALTnames: | | anti-DKFZp686N23123 antibody, anti-ER alpha antibody, anti-ER antibody, anti-Era antibody, anti-ESR 1 antibody, anti-ESR antibody, anti-ESR1 antibody, anti-ESRA antibody, anti-Estradiol receptor antibody, anti-Estrogen receptor 1 antibody, anti-Estrogen receptor antibody, anti-Major ORF antibody, anti-NR3A1 antibody |
| ProteinTarget: | | Estrogen Receptor |
| PackageSize: | | 0.1 mg |
| GeneralRef: | | General / background references:Mueller SO & Korach KS Estrogen receptors and endocrine diseases: lessons from estrogen receptor knockout mice. Curr Opin Pharmacol 1:613-9 (2001). Olefsky JM Nuclear receptor minireview series. J Biol Chem 276:36863-4 (2001). Moggs JG & Orphanides G Estrogen receptors: orchestrators of pleiotropic cellular responses. EMBO Rep 2:775-81 (2001). Wyckoff MH et al. Plasma membrane estrogen receptors are coupled to endothelial nitric-oxide synthase through Galpha(i). J Biol Chem 276:27071-6 (2001). Couse JF et al. Tissue distribution and quantitative analysis of estrogen receptor-alpha (ERalpha) and estrogen receptor-beta (ERbeta) messenger ribonucleic acid in the wild-type and ERalpha-knockout mouse. Endocrinology 138:4613-21 (1997). |
| more information: | | company product webpage |
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