| CatalogCode: | NB120-13532 |
| ProductName: | Hsp60 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp60 (Mab11-13) |
| Clone: | Mab11-13 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant fragment, amino acids 31-547 (Human). |
| Epitope: | This antibody recognizes a surface epitope of Hsp60 in the region of aminoacids 288-366. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Dog, Dolphin, Drosophilia melanogaster, Guinea pig, Hamster, Monkey, Pig, Rabbit, Rainbow trout and Snake. Does not cross-react with E. coli or S. cerevisiae. Not yet tested in other species. |
| Packaging: | 0.1 ml protein G purified Mouse ascites. |
| Uses: | FACS: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. WB: Use at a dilution of 1/10,000. Detects a band of approximately 60 kDa (predicted molecular weight: 68.8 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Mitochondrial/chloroplast |
| Control: | HeLa Heat Shocked Cell Lysate or Recombinant Human Hsp60 Protein |
| Background: | Heat shock protein 60 (Hsp60) is a molecular chaperone that participates in the folding and assembly of mitochondrial proteins and facilitates proteolytic degradation of misfolded or denatured proteins. Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (also known as Rubisco binding protein), GroEL, the E. coli Hsp60, and Hsp65, a major antigen of mycobacteria. Hsp60 is strongly dependent upon its co-chaperone, Hsp10 that binds the molecule and regulates its substrate binding and ATPase activity. Hsp60 is rapidly upregulated by a range of cellular insults including oxidative stress, viral infection, chemical exposure, increased temperature and irradiation. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | 50% Glycerol, PBS, 0.1mM PMSF. pH 7.2 |
| ListPrice: | 295 |
| AppSummary: | FACS, IHC, WB |
| SpeciesSummary: | Bv, Ca, Dr, Ha, Hu, Mu, Po, Mk, Rt, Rb, Gp, Fi |
| ProteinTarget: | Hsp60 |
| PackageSize: | 0.1 ml |
| GeneralRef: | Singh AK & Lakhotia SC Tissue-specific variations in the induction of Hsp70 and Hsp64 by heat shock in insects. <i>Cell Stress Chaperones</i> <b>5</b>:90-7 (2000). <PUBMED:11147970> Nielsen KL <i>et al.</i> A single-ring mitochondrial chaperonin (Hsp60-Hsp10) can substitute for GroEL-GroES in vivo. <i>J Bacteriol</i> <b>181</b>:5871-5 (1999). <PUBMED:10482535> Samali A <i>et al.</i> Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells. <i>EMBO J</i> <b>18</b>:2040-8 (1999). <PUBMED:10205158> Soltys BJ & Gupta RS Cell surface localization of the 60 kDa heat shock chaperonin protein (hsp60) in mammalian cells. <i>Cell Biol Int</i> <b>21</b>:315-20 (1997). <PUBMED:9243807> Jindal S <i>et al.</i> Primary structure of a human mitochondrial protein homologous to the bacterial and plant chaperonins and to the 65-kilodalton mycobacterial antigen. <i>Mol Cell Biol</i> <b>9</b>:2279-83 (1989). <PUBMED:2568584> |
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