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| Catalog Code: | NB120-13508 |
| Product Name: | ERp72 Antibody |
| Product Description: | Rabbit Polyclonal anti-ERp72 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: (C)FIDEHATKRSRTKEEL, with Cysteine added, conjugated to KLH, corresponding to amino acids 623-638 of Mouse ERp72. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Dog, Guinea pig, Hamster, Monkey and Rabbit. Not yet tested in other species. |
| Packaging: | 0.05 ml Whole Rabbit antisera. |
| Uses: | In Western Blot detects a band of approximately 72 kDa. Optimal working dilutions should be determined by investigator. |
| Localization: | Endoplasmic reticulum |
| Background: | ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 75mM Sodium Chloride, 5mM EDTA, 25mM Sodium Borate, 100mM Borate. |
| Application Summary: | Western Blot 1:1,000 |
| Species Summary: | Bv, Ca, Ha, Hu, Mu, Mk, Rt, Rb, Gp, Gt, |
| Alternate Names: | anti-Calcium binding protein intestinal related antibody, anti-ERP 70 antibody, anti-ERp 72 antibody, anti-ERP70 antibody, anti-PDIA 4 antibody, anti-PDIA4 antibody, anti-PDIR antibody, anti-Protein disulfide isomerase A4 antibody, anti-Protein disulfide isomerase associated 4 antibody, anti-Protein disulfide isomerase family A member 4 antibody, anti-Protein disulfide isomerase related protein antibody, anti-Protein ERp 72 antibody, anti-Protein ERp72 antibody |
| Package Size: | 0.05 ml |
| Preservative: | No Preservative |
| Notes Main: | For maximum product recovery, after thawing, centrifuge the product vial before removing cap. |
| General References: | This product has been used in:Taghibiglou C et al. Hepatic very low density lipoprotein-ApoB overproduction is associated with attenuated hepatic insulin signaling and overexpression of protein-tyrosine phosphatase 1B in a fructose-fed hamster model of insulin resistance. J Biol Chem 277:793-803 (2002). Sharma M et al. Conformational and temperature-sensitive stability defects of the delta F508 cystic fibrosis transmembrane conductance regulator in post-endoplasmic reticulum compartments. J Biol Chem 276:8942-50 (2001). Kobayashi T et al. Abundant expression of 150-kDa oxygen-regulated protein in mouse pancreatic beta cells is correlated with insulin secretion. Biochem Biophys Res Commun 267:831-7 (2000). General / background references:Mazzarella RA et al. ERp72, an abundant luminal endoplasmic reticulum protein, contains three copies of the active site sequences of protein disulfide isomerase. J Biol Chem 265:1094-101 (1990). |
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