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| Catalog Code: | NB120-13507 |
| Product Name: | ERp57 Antibody |
| Product Description: | Rabbit Polyclonal anti-ERp57 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | ERp57 from rat liver |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Cat, Dog, Guinea pig, Hamster, Monkey, Pig and Rabbit. Not yet tested in other species. |
| Packaging: | 0.05 ml protein A purified Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/1000. This dilution detects Erp57 in 20ug of rat liver microsomal preparation by ECL analysis. Predicted molecular weight: 57 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Endoplasmic reticulum |
| Background: | ERp57 (also known as Grp58 and ER-60) is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs. It functions as an accessory oxidoreductase involved in disulfide bond formation. As an ER resident protein, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their proline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that posses s mono-glucosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hemagglutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub -optimal peptides and reduced expression and stability on the cell surface. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS, pH 7.2, 50% glycerol |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Disulfide isomerase ER 60 antibody, anti-ERp60 antibody, anti-ERp61 antibody, anti-Glucose Regulated Protein 58 Kd antibody, anti-GRP57 antibody, anti-GRP58 antibody, anti-Phospholipase C alpha antibody, anti-PI PLC antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.09% Sodium azide |
| General References: | General / background references:Nigam SK et al. A set of endoplasmic reticulum proteins possessing properties of molecular chaperones includes Ca(2+)-binding proteins and members of the thioredoxin superfamily. J Biol Chem 269:1744-9 (1994). Camp LA & Hofmann SL Purification and properties of a palmitoyl-protein thioesterase that cleaves palmitate from H-Ras. J Biol Chem 268:22566-74 (1993). Mobbs CV et al. HIP-70: an isoform of phosphoinositol-specific phospholipase C-alpha. Science 249:566-7 (1990). |
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