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| Catalog Code: | NB120-13497 |
| Product Name: | alpha B Crystallin Antibody |
| Product Description: | Rabbit Polyclonal anti-alpha B Crystallin |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: REEKPAVTAAPKK conjugated to KLH, corresponding to amino acids 163-175 of Human alpha B Crystallin. |
| Specificity: | This antibody does not cross-react with beta Crystallin nor alpha A Crystallin. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Cow and Pig. Expected to cross-react with Hamster (100% identity with immunogen) and Rabbit (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Whole Rabbit antisera. |
| Uses: | WB: Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa). |
| Background: | Lens proteins consist almost entirely of Crystallins (about 95%). Crystallins are also found in vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha Crystallin is the largest of the crystallins and is composed of 2 primary gene products; alpha A and alpha B. There are at least 5 different proteins comprising the beta Crystallins. The gamma Crystallins are monomeric, but there are at least 5 gamma Crystallins identified in bovine and rat lens. Alpha Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha Crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha B Crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Constituents: Whole Serum |
| Application Summary: | immunoprecipitation , Western Blot 1/1000 - 1/2000, Immunocytochemistry 1/200 |
| Species Summary: | Bv, Hu, Mu, Po, Rt, |
| Alternate Names: | anti-Alpha crystallin B chain antibody, anti-AACRYA antibody, anti-Alpha crystallin B chain antibody, anti-Alpha(B) crystallin antibody, anti-CRYA2 antibody, anti-CRYAB antibody, anti-Crystallin alpha B antibody, anti-Crystallin alpha polypeptide 2 antibody, anti-CTPP 2 antibody, anti-CTPP2 antibody, anti-Heat shock 20 kD like protein antibody, anti-Heat shock protein beta 5 antibody, anti-HSPB5 antibody, anti-NY REN 27 antigen antibody, anti-Renal carcinoma antigen NY REN 27 antibody, anti-Rosenthal fiber component antibody |
| Package Size: | 0.1 ml |
| Preservative: | No Preservative |
| Notes Main: | For maximum product recovery, after thawing, centrifuge the product vial before removing cap |
| General References: | This product has been used in:Golenhofen N et al. Expression and induction of the stress protein alpha-B-crystallin in vascular endothelial cells. Histochem Cell Biol 117:203-9 (2002). Harris MB & Starnes JW Effects of body temperature during exercise training on myocardial adaptations. Am J Physiol Heart Circ Physiol 280:H2271-80 (2001). Yang J et al. Decreased SLIM1 expression and increased gelsolin expression in failing human hearts measured by high-density oligonucleotide arrays. Circulation 102:3046-52 (2000). Suzuki A et al. MKBP, a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase. J Cell Biol 140:1113-24 (1998). |
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