| request information: |
|
| Catalog Code: | NB120-13491 |
| Product Name: | Hsp20 Antibody |
| Product Description: | Rabbit Polyclonal anti-Hsp20 |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1.0 mg/ml |
| Immunogen: | Rat skeletal muscle Hsp20. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Cat, Dog, Guinea pig, Pig, Rabbit and Sheep. Not yet tested in other species. |
| Packaging: | 0.05 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Detects a band of approximately 20 kDa. |
| Background: | Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 3% BSA, 0.1M Phosphate. pH 7.0 |
| Application Summary: | immunoprecipitation 12.5 ug/ml, Western Blot 0.05 ug/ml |
| Species Summary: | Bv, Ca, Hu, Mu, Rt, Rb, Fe, Sh, |
| Alternate Names: | anti-FLJ32389 antibody, anti-Heat shock protein alpha-crystallin-related B6 antibody, anti-Heat shock protein beta 6 antibody, anti-Heat-shock 20 kDa like-protein p20 antibody, anti-Hspb6 antibody |
| Package Size: | 0.05 mg |
| Preservative: | No Preservative |
| General References: | General / background references:Tessier DJ et al. The small heat shock protein (HSP) 20 is dynamically associated with the actin cross-linking protein actinin. J Surg Res 111:152-7 (2003). Kozawa O et al. HSP20, low-molecular-weight heat shock-related protein, acts extracellularly as a regulator of platelet functions: a novel defense mechanism. Life Sci 72:113-24 (2002). Beall A et al. The small heat shock-related protein, HSP20, is phosphorylated on serine 16 during cyclic nucleotide-dependent relaxation. J Biol Chem 274:11344-51 (1999). Brophy CM et al. Phosphorylation of the small heat shock-related protein, HSP20, in vascular smooth muscles is associated with changes in the macromolecular associations of HSP20. J Biol Chem 274:6324-9 (1999). Beall AC et al. Cyclic nucleotide-dependent vasorelaxation is associated with the phosphorylation of a small heat shock-related protein. J Biol Chem 272:11283-7 (1997). Kato K et al. Purification and characterization of a 20-kDa protein that is highly homologous to alpha B crystallin. J Biol Chem 269:15302-9 (1994). |
order or
more information: | company product webpage |
|