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| Catalog Code: | NB120-13487 |
| Product Name: | DC-SIGN Antibody |
| Product Description: | Mouse Monoclonal anti-DC-SIGN (120507) |
| Clone: | 120507 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Tissue / cell preparation (NIH/3T3 transfectants stably expressing human DC-SIGN1). |
| Specificity: | The antibody does not react with parental mouse cells or irrelevant transfectants, such as human DC-SIGN2. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg purified Mouse IgG. |
| Uses: | FACS: Use at a concentration of 25 - 50 ug/ml (10 ul of a 25-50 ug/ml stock solution of the monoclonal antibody is mixed with up to 1 million cells in a minimal volume (< 0.2 ml) of buffer (PBS + 0.5% BSA). The reaction is incubated at room temperature for 30 minutes. Inhib: Use at a concentration of 5 ug/ml. (This antibody shows inhibition of DC-SIGN expressing NIH-3T3 cell adhesion to ICAM-3 coated 96 well plates. The adhesion of NIH-3T3-DC-SIGN cells (5 x 104 cells/well) to plates coated with 5 ug/ml (100 ul/well) of recombinant human ICAM-3/Fc is maximally inhibited (100%) using 100 ul of a 5 ug/ml antibody solution per well. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cell Membrane and Secreted |
| Background: | Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes, where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV1, HIV2 and SIV bind to DCs via DCSIGN. ICAM3 is the natural ligand for DC-SIGN. A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DCSIGN2) was identified recently. DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR. The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG2b |
| Host Name: | Mouse |
| Buffer: | Preservative: 0.08% Sodium Azide Constituents: PBS. pH 7.4 |
| Application Summary: | flow cytometry / FACS analysis, IA |
| Species Summary: | Hu |
| Alternate Names: | anti-C type lectin domain family 4 member L antibody, anti-CD 209 antibody, anti-CD209 antibody, anti-CD209 antigen antibody, anti-CD209 molecule antibody, anti-CDSIGN antibody, anti-CLEC4L antibody, anti-DC SIGN1 antibody, anti-DCSIGN antibody, anti-Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody, anti-Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody, anti-Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody, anti-HIV GP120 Binding Protein antibody, anti-MGC129965 antibody |
| Package Size: | 0.1 mg |
| General References: | General / background references:Geijtenbeek TB et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 100:575-85 (2000). Geijtenbeek TB et al. DC-SIGN-ICAM-2 interaction mediates dendritic cell trafficking. Nat Immunol 1:353-7 (2000). Geijtenbeek TB et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 100:587-97 (2000). |
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