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| Catalog Code: | NB120-13258 |
| Product Name: | Synapsin II Antibody |
| Product Description: | Rabbit Polyclonal anti-Synapsin II |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | Synthetic peptide: ASPGPERRPPPAQA (Rat) conjugated to KLH. This sequence is identical in synapsin IIa and IIb. |
| Specificity: | This antibody detects ~74 and ~54 kDa proteins, corresponding to the apparent molecular masses of synapsin IIa and IIb on SDS-PAGE immunoblots, in samples from mouse and rat origins. |
| Cross Reactivity: | Cross-reacts with Mouse and Rat.Expected to cross-react with Human (92% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml protein A purified Rabbit IgG |
| Uses: | WB: Use at a dilution of 1/5000. This dilution was sufficient for detection of Synapsin II in 15 ug of rat brain tissue extract. Detects bands of approximately 74 and 54 kDa, corresponding to the apparent molecular masses of synapsin IIa and IIb.(predicted molecular weight: 57.5 kDa). Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Synapse |
| Background: | Synapsin II is a member of the synapsin gene family. Synapsins are neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family is a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | phosphate buffered saline (PBS), pH 7.2, 50% glycerol |
| Application Summary: | Western Blot |
| Species Summary: | Mu, Rt, |
| Alternate Names: | anti-SYN2 antibody, anti-Synapsin 2 antibody, anti-Synapsin II isoform IIa antibody, anti-Synapsin II isoform IIb antibody, anti-Synapsin2 antibody, anti-SynapsinII antibody, anti-SYNII antibody, anti-SYNIIa antibody, anti-SYNIIb antibody |
| Package Size: | 0.05 ml |
| Preservative: | 0.09% Sodium azide |
| General References: | General / background references:Nielander HB et al. Phosphorylation-dependent effects of synapsin IIa on actin polymerization and network formation. Eur J Neurosci 9:2712-22 (1997). Rosahl TW et al. Essential functions of synapsins I and II in synaptic vesicle regulation. Nature 375:488-93 (1995). Ferreira A et al. Aberrant neurites and synaptic vesicle protein deficiency in synapsin II-depleted neurons. Science 264:977-9 (1994). |
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more information: | company product webpage |