| CatalogCode: | NB120-12421 |
| ProductName: | Cytohesin 2 Antibody |
| Product Description: | Mouse Monoclonal anti-Cytohesin 2 (CYT2-21) |
| Clone: | CYT2-21 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant full length protein (Human). |
| Specificity: | Ab12421 does not cross-react with other members of the cytohesin family. |
| CrossReactivity: | Cross-reacts with Human and Rat.Not yet tested in other species. |
| Packaging: | 0.05 ml purified Mouse IgG. |
| Uses: | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. WB: Use at a concentration of 5 - 10 ug/ml, this concentration is determined using a whole extract of rat brain. Predicted molecular weight: 40 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Plasma membrane |
| Control: | Whole extract of rat brain. |
| Background: | The ADP-ribosylation factor (Arf) family comprises a group of structurally and functionally conserved 21 kDa proteins, which are members of the Ras superfamily of regulatory GTP-binding proteins. Arf is involved in intracellular protein traffic to and within the Golgi complex. Arf has a number of disparate activities including maintenance of organelle integrity, assembly of coat proteins, as a co-factor for cholera toxin and as an activator of phospholipase D. Like other small GTPases, Arf is found to be active when bound to GTP and inactive when bound to GDP. Arf's activation is dependent upon guanine nucleotide exchange factors (GEFs) which increase the rate of exchange of bound GDP with GTP. All GEFs have a highly conserved Sec7 domain. GEF activity lies in the Sec7 domain and this activity has been shown to be inhibited by the fungal metabolite brefeldin-A (BFA). A small group of GEFs which are insensitive to brefeldin-A (BFA) include cytohesin-1 (B2-1), cytohesin-2 (ARNO), cytohesin-3 (ARNO3), and cytohesin-4. All cytohesins function in the cell periphery and contain a pleckstrin homology (PH) domain. The PH domain has been shown to interact with phosphatidylinositol 3,4,5-triphosphate and is believed to promote membrane targeting of the cytohesins. Recruitment of the cytohesins to the membranes can occur in response to tyrosine kinase receptor activation. This response appears to require the activation of phosphatidylinositol 3-kinase (PI 3-kinase). |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 0.01M PBS. pH 7.4 |
| ListPrice: | 285 |
| AppSummary: | ELISA, ICC, WB |
| ALTnames: | anti-ARF exchange factor antibody, anti-ARF nucleotide-binding site opener antibody, anti-ARNO protein antibody, anti-CTS18.1 antibody, anti-Cytohesin 2 antibody, anti-Pleckstrin homology Sec7 and coiled-coil domains 2 antibody, anti-PSCD2 antibody, anti-PSCD2L antibody, anti-Sec7p-L antibody, anti-Sec7pL antibody |
| ProteinTarget: | Cytohesin 2 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Maranda B et al. Intra-endosomal pH-sensitive recruitment of the Arf-nucleotide exchange factor ARNO and Arf6 from cytoplasm to proximal tubule endosomes. J Biol Chem 276:18540-50 (2001). Monier S et al. Overexpression of the ARF1 exchange factor ARNO inhibits the early secretory pathway and causes the disassembly of the Golgi complex. J Cell Sci 111 ( Pt 22):3427-36 (1998). Venkateswarlu K et al. Insulin-dependent translocation of ARNO to the plasma membrane of adipocytes requires phosphatidylinositol 3-kinase. Curr Biol 8:463-6 (1998). Frank S et al. ARNO is a guanine nucleotide exchange factor for ADP-ribosylation factor 6. J Biol Chem 273:23-7 (1998). |
| NotesMain: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
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