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| Catalog Code: | NB120-12364 |
| Product Name: | Agrin Antibody |
| Product Description: | Mouse Monoclonal anti-Agrin (Agr 247) |
| Clone: | Agr 247 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Recombinant rat agrin, C-terminal construct. |
| Epitope: | Ab12364 binds to an epitope close to splicing site Z and causes a marked reduction in the number of nicotinic acetylcholine receptor(nAChR) clusters in samples from mouse and rat origins. |
| Specificity: | Ab12364 regognises agrin. |
| Cross Reactivity: | Cross-reacts with Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse IgG. |
| Uses: | ICC: Use at a concentration of 2 ug/ml. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Synaptic basal lamina at the neuromuscular junction. |
| Background: | Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | 50% Glycerol, PBS, 0.1mM PMSF |
| Application Summary: | Immunocytochemistry, |
| Species Summary: | Mu, Rt, |
| Alternate Names: | anti-AGRN antibody, anti-FLJ45064 antibody, anti-OTTHUMP00000044043 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.09% Sodium azide |
| General References: | General / background references:Khan AA et al. Physiological regulation of the immunological synapse by agrin. Science 292:1681-6 (2001). Hoch W Formation of the neuromuscular junction. Agrin and its unusual receptors. Eur J Biochem 265:1-10 (1999). Hoch W et al. Structural domains of agrin required for clustering of nicotinic acetylcholine receptors. EMBO J 13:2814-21 (1994). Ferns MJ et al. The ability of agrin to cluster AChRs depends on alternative splicing and on cell surface proteoglycans. Neuron 11:491-502 (1993). Tsim KW et al. cDNA that encodes active agrin. Neuron 8:677-89 (1992). Wallace BG Agrin-induced specializations contain cytoplasmic, membrane, and extracellular matrix-associated components of the postsynaptic apparatus. J Neurosci 9:1294-302 (1989). |
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