| CatalogCode: | NB120-12358 |
| ProductName: | Agrin Antibody |
| Product Description: | Mouse Monoclonal anti-Agrin (Agr 33) |
| Clone: | Agr 33 |
| Clonality: | Monoclonal |
| Immunogen: | Recombinant rat agrin, C-terminal construct. |
| Epitope: | Epitope has been mapped near the 2nd laminin domain homology region and causes a marked reduction in the number of nicotinic acetylcholine receptor (nAChR) clusters. Insertion of an 8aa peptide at splicing site Z increases the clustering activity |
| Specificity: | Ab12358 detects all isoforms of agrin. |
| CrossReactivity: | Cross-reacts with Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.05 mg protein G purified Mouse IgG. |
| Uses: | ICC: Use at a concentration of 2 ug/ml. WB: Use at a concentration of 2 ug/ml. Predicted molecular weight: 208.6 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Synaptic basal lamina at the neuromuscular junction. |
| Background: | Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS, pH 7.2, 50% glycerol |
| ListPrice: | 285 |
| AppSummary: | ICC, WB |
| SpeciesSummary: | Mu, Rt |
| ALTnames: | anti-AGRN antibody, anti-FLJ45064 antibody, anti-OTTHUMP00000044043 antibody |
| ProteinTarget: | Agrin |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Khan AA et al. Physiological regulation of the immunological synapse by agrin. Science 292:1681-6 (2001). Hoch W Formation of the neuromuscular junction. Agrin and its unusual re |
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