| CatalogCode: | NB120-12225 |
| ProductName: | PDI Antibody |
| Product Description: | Mouse Monoclonal anti-PDI (1D3) |
| Clone: | 1D3 |
| Clonality: | Monoclonal |
| Immunogen: | Peptide corresponding to amino acids 499-509 of rat protein disulfide isomerase conjugated to KLH. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Xenopus laevis, Cow, Chicken, Dog, Guinea pig, Hamster, Monkey, Pig and Sheep.Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse IgG. |
| Uses: | ICC: Use at a concentration of 2 - 5 ug/ml. IP: Use at a concentration of 10 ug/ml. WB: Use at a concentration of 2 ug/ml. Predicted molecular weight: 58 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Background: | The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS, pH 7.2, 0.09% azide, 50% glycerol |
| ListPrice: | 295 |
| AppSummary: | ICC, IP, WB |
| ALTnames: | anti-Disulphide Isomerase antibody, anti-GIT antibody, anti-Glutathione Insulin Transhydrogenase antibody, anti-Procollagen Proline 2 Oxoglutarate 4 Dioxygenase beta antibody, anti-Protein Disulphide Isomerase antibody,, anti- Thyroid Hormone Binding Protein p55 antibody |
| ProteinTarget: | PDI ER Marker |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Vaux, D., et al. (1990) Nature 345, 495-502. 2. Ferrari, D.M. et al. (1999) Biochem J. 339, 1-10. 3. Essex, D.W. et al. (1999) Br J Haematol. 104, 448-454. 4. O'Farrell, C., et al. (2002) Neurosci Lett. 327, 75-78. 5. Huovila, A-P., et al. (1992) J Cell Biol. 118, 1305-1320. 6. Mehtani, S., et al. (1998) J Biol Chem. 273, 13236-13244. 7. Walker, R.H., et al. (2002) Neurology 58, 120-124. |
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