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| Catalog Code: | NB120-12192 |
| Product Name: | PRMT6 Antibody |
| Product Description: | Rabbit Polyclonal anti-PRMT6 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 0.9 mg/ml |
| Immunogen: | Synthetic peptide: SQPKKRKLESGGGGE conjugated to KLH via a C-terminal added cysteine, corresponding to amino acids 2-16 of Human PRMT6. |
| Specificity: | Anti PRMT6 (SE-15) antibody reacts specifically with PRMT6. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IF: Use at a concentration of 3 - 4 ug/ml (recommended using paraformaldehyde fixed HeLa cells). IP: 2.5 - 5 ug of the antibody immunoprecipitates PRMT6 protein from extracts of PRMT6 transfected 293T cells. WB: Use at a concentration of 0.25 - 0.5 ug/ml (recommended using cell extracts of PRMT6 transfected 293T cells). Staining of the PRMT6 band in immunoblotting is specifically inhibited by the PRMT6 immunizing peptide. Predicted molecular weight: 38 kDa.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | PRMT6 is a protein arginine N-methyltransferase, and catalyzes the sequential transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation is a prevalent post-translational modification in eukaryotic cells that has been implicated in signal transduction, the metabolism of nascent pre-RNA, and the transcriptional activation processes. IPRMT6 is functionally distinct from two previously characterized type I enzymes, PRMT1 and PRMT4. In addition, PRMT6 displays automethylation activity; it is the first PRMT to do so. PRMT6 has been shown to act as a restriction factor for HIV replication. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
| Application Summary: | immunofluorescence, immunoprecipitation, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-PRMT6 antibody, anti-PRMT-6 antibody, anti-Protein Arginine Methyltransferase 6 antibody, anti-HRMT1L6 antibody, anti-HMT1 hnRNP Methyltransferase-like |
| Package Size: | 0.1 ml |
| Notes Main: | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| General References: | General / background references:Frankel A et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J Biol Chem 277:3537-43 (2002). Davie JK & Dent SY Transcriptional control: an activating role for arginine methylation. Curr Biol 12:R59-61 (2002). Rho J et al. Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family. J Biol Chem 276:11393-401 (2001). Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). Frankel A & Clarke S PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain. J Biol Chem 275:32974-82 (2000). |
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