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| Catalog Code: | NB120-12189 |
| Product Name: | PRMT1 Antibody |
| Product Description: | Mouse Monoclonal anti-PRMT1 (PRMT1-171) |
| Clone: | PRMT1-171 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 2 mg/ml |
| Immunogen: | Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length PRMT1. |
| Specificity: | This antibody reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5. |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Rat, Cow, Chicken, Dog, Hamster and Monkey.Not yet tested in other species. |
| Packaging: | 0.05 ml purified Mouse IgG. |
| Uses: | DID: Use at an assay dependent dilution. ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2 ug/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 0.01M PBS. pH 7.4 |
| Application Summary: | RID, ELISA, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, Bv, Ch, Ca, Ha, Mk |
| Alternate Names: | anti-PRMT1 antibody, anti-PRMT-1 antibody, anti-Protein Arginine N-Methyltransferase 1 antibody, anti-HRMT1L2 antibody, anti-HMT1 hnRNP Methyltransferase-like 2 antibody, anti-ANM1 antibody, anti-Arginine N-Methyltransferase 1 antibody, anti-IR1B4 antibody, anti-Interferon Receptor 1-Bound Protein 4 antibody, anti-H |
| Package Size: | 0.05 ml |
| Notes Main: | If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| General References: | General / background references:Cata J et al. Sam68 RNA binding protein is an in vivo substrate for protein arginine N-methyltransferase 1. Mol Biol Cell 14:274-87 (2003). Frankel A et al. The novel human protein arginine N-methyltransferase PRMT6 is a nuclear enzyme displaying unique substrate specificity. J Biol Chem 277:3537-43 (2002). Rho J et al. Prmt5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family. J Biol Chem 276:11393-401 (2001). Frankel A & Clarke S PRMT3 is a distinct member of the protein arginine N-methyltransferase family. Conferral of substrate specificity by a zinc-finger domain. J Biol Chem 275:32974-82 (2000). |
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