| CatalogCode: | NB120-12182 |
| ProductName: | Histone H3 Antibody |
| Product Description: | Rabbit Polyclonal anti-Histone H3 [Ser10] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide conjugated to KLH, corresponding to amino acids 7-20 of Human Histone H3, phosphorylated at S10. |
| Specificity: | Staining of (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-phosphorylated histone H3 peptide. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse, Rat, Xenopus laevis, Cow, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to perfect or near-perfect sequence identity.Not yet tested in other species. |
| Packaging: | 0.05 ml IgG purified Rabbit antisera. |
| Uses: | Indirect IF: Use at a dilution of 1/500 using human epitheloid carcinoma HeLa cell line treated with nocodazole. WB: Use at a dilution of 1/2000. Detects a band of approximately 17 kDa using whole cell extract of human acute T cell leukemia Jurkat cell line treated with nocodazole. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.We advise you to centrifuge this product vial before use. |
| Localization: | Nuclear |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 15mM Sodium AzideConstituents: 0.01M PBS. pH 7.4 |
| ListPrice: | 330 |
| AppSummary: | IF, WB |
| ALTnames: | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti-H3/l antibody, anti-Histone H3 antibody |
| ProteinTarget: | Histone H3 (phospho S10) |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Crosio C et al. Mitotic phosphorylation of histone H3: spatio-temporal regulation by mammalian Aurora kinases. Mol Cell Biol 22:874-85 (2002). Hsu JY et al. Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes. Cell 102:279-91 (2000). Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). Thomson S et al. The nucleosomal response associated with immediate-early gene induction is mediated via alternative MAP kinase cascades: MSK1 as a potential histone H3/HMG-14 kinase. EMBO J 18:4779-93 (1999). Thomson S et al. MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction. Semin Cell Dev Biol 10:205-14 (1999). Chadee DN et al. Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts. J Biol Chem 274:24914-20 (1999). Sassone-Corsi P et al. Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of histone H3. Science 285:886-91 (1999). Kornberg RD & Lorch Y Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98:285-94 (1999). Struhl K Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12:599-606 (1998). De Cesare D et al. Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene. Proc Natl Acad Sci U S A 95:12202-7 (1998). Barratt MJ et al. Mitogen-stimulated phosphorylation of histone H3 is targeted to a small hyperacetylation-sensitive fraction. Proc Natl Acad Sci U S A 91:4781-5 (1994). Mahadevan LC et al. Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors. Cell 65:775-83 (1991). |
| Phosphorylated: | Ser10 |
| NotesMain: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
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