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| Catalog Code: | NB120-12181 |
| Product Name: | Histone H3 Antibody |
| Product Description: | Rabbit Polyclonal anti-Histone H3 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Immunogen: | synthetic acetylated and phosphorylated [Ac-Lys9, pSer10] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3. |
| Specificity: | Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide. |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with Mouse, Rat, Xenopus laevis, Cow, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity.Not yet tested in other species. |
| Packaging: | 0.5 ml |
| Uses: | ChIP:Approximately 4-5 ug of each antibody was used with 30-40 ug of chromatin per ChIP.IF: Use at a dilution of 1/1000. WB: Use at a dilution of 1/1000. Detects a band of approximately 17 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.We advise you to centrifuge this product vial before use. |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Phosphorylation, particularly that of histones H1 and H3, has long been implicated in chromosome condensation during mitosis. However, converging evidence suggests that H3 phosphorylation (specifically serine 10) is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. The potential importance of the serine 10 phosphorylation mark in H3 is strengthened by the finding that MSK1, a kinase activated by growth factor and stress stimuli, also phosphorylates H3 in vitro. H3 phosphorylation at serine 10 in conjunction with phosphorylation at serine 28, is also required for proper segregation and condensation of chromosomes during mitosis and meiosis. |
| Purity: | IgG purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Constituents: 0.01M PBS |
| Application Summary: | chromatin immunoprecipitation, immunofluorescence, Western Blot |
| Species Summary: | Hu |
| Alternate Names: | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti-H3/l antibody, anti-Histone H3 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 15mM Sodium Azide |
| Notes Main: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| General References: | General / background references:Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). Kornberg RD & Lorch Y Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98:285-94 (1999). Thomson S et al. MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction. Semin Cell Dev Biol 10:205-14 (1999). Chadee DN et al. Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts. J Biol Chem 274:24914-20 (1999). Sassone-Corsi P et al. Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of histone H3. Science 285:886-91 (1999). Thomson S et al. The nucleosomal response associated with immediate-early gene induction is mediated via alternative MAP kinase cascades: MSK1 as a potential histone H3/HMG-14 kinase. EMBO J 18:4779-93 (1999). De Cesare D et al. Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene. Proc Natl Acad Sci U S A 95:12202-7 (1998). Struhl K Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12:599-606 (1998). Zhang W et al. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J 17:3155-67 (1998). Kuo MH et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature 383:269-72 (1996). Sobel RE et al. Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A 92:1237-41 (1995). Barratt MJ et al. Mitogen-stimulated phosphorylation of histone H3 is targeted to a small hyperacetylation-sensitive fraction. Proc Natl Acad Sci U S A 91:4781-5 (1994). Mahadevan LC et al. Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors. Cell 65:775-83 (1991). |
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