| CatalogCode: | | NB120-12180 |
| ProductName: | | Histone H3 Antibody |
| Product Description: | | Mouse Monoclonal anti-Histone H3 (APH3-64) [Lys9/Ser10] |
| Clone: | | APH3-64 |
| Clonality: | | Monoclonal |
| Immunogen: | | A synthetic, acetylated and phosphorylated histone H3 peptide (amino acids 7-20, acetyl K9 & phospho S10) corresponding to the N-terminus of human histone H3, conjugated to KLH. |
| Specificity: | | Staining of (acetyl K9) & (phospho S10) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide or with singly modified (acetyl K9) or (phospho S10) histone H3 peptide. |
| CrossReactivity: | | Cross-reacts with Human. Expected to cross-react with Mouse, Rat, Xenopus laevis, Cow, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity across a wide variety of species. |
| Packaging: | | 0.05 ml Immunogen affinity purified Mouse ascites. |
| Uses: | | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 2 ug/ml. Detects a band of approximately 17 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.We advise you to centrifuge this product vial before use. |
| Localization: | | Nuclear |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG2a |
| Host_Name: | | Mouse |
| Buffer: | | Preservative: 15mM Sodium AzideConstituents: 0.01M PBS |
| ListPrice: | | 330 |
| AppSummary: | | ELISA, IP, WB |
| ALTnames: | | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti-H3/l antibody, anti-Histone H3 antibody |
| ProteinTarget: | | Histone H3 (acetyl K9) & (phospho S10) |
| PackageSize: | | 0.05 ml |
| GeneralRef: | | General / background references:Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). Kornberg RD & Lorch Y Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98:285-94 (1999). Thomson S et al. MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction. Semin Cell Dev Biol 10:205-14 (1999). Chadee DN et al. Increased Ser-10 phosphorylation of histone H3 in mitogen-stimulated and oncogene-transformed mouse fibroblasts. J Biol Chem 274:24914-20 (1999). Sassone-Corsi P et al. Requirement of Rsk-2 for epidermal growth factor-activated phosphorylation of histone H3. Science 285:886-91 (1999). Thomson S et al. The nucleosomal response associated with immediate-early gene induction is mediated via alternative MAP kinase cascades: MSK1 as a potential histone H3/HMG-14 kinase. EMBO J 18:4779-93 (1999). De Cesare D et al. Rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of CREB protein and transcription of c-fos gene. Proc Natl Acad Sci U S A 95:12202-7 (1998). Struhl K Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12:599-606 (1998). Zhang W et al. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J 17:3155-67 (1998). Kuo MH et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature 383:269-72 (1996). Sobel RE et al. Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A 92:1237-41 (1995). Barratt MJ et al. Mitogen-stimulated phosphorylation of histone H3 is targeted to a small hyperacetylation-sensitive fraction. Proc Natl Acad Sci U S A 91:4781-5 (1994). Mahadevan LC et al. Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors. Cell 65:775-83 (1991). |
| Phosphorylated: | | Lys9/Ser10 |
| NotesMain: | | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| more information: | | company product webpage |