| Catalog Code: | NB120-12179 |
| Product Type: | Primary Antibody |
| Product Name: | Histone H3 Antibody |
| List Price: | 325 |
| Clonality: | Monoclonal |
| Gene ID: | 3020 |
| Host: | Mouse |
| Clone: | AH3-120 |
| Isotype: | IgG1 |
| Product Description: | Mouse Monoclonal anti-Histone H3 (AH3-120) [Lys9] |
| Phosphorylated: | Lys9 |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with Mouse, Rat, Xenopus laevis, Cow, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity across many species. Not yet tested in oth |
| Species Summary: | Bv, Ch, Dr, Hu, Mu, Rt, Ce, Xp |
| Alternate Names: | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti |
| Immunogen: | A synthetic, acetylated histone H3 peptide (amino acids 7-20, acetyl K9) corresponding to the N-terminus of human histone H3, conjugated to KLH. |
| Localization: | Nuclear |
| Specificity: | Staining of (acetyl K9) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide. |
| Purity: | Immunogen affinity purified |
| Buffer: | Constituents: 0.01M PBS. pH 7.4 |
| Packaging: | 0.05 ml Immunogen affinity purified Mouse ascites. |
| Package Size: | 0.05 ml |
| Concentration: | 2.1 mg/ml |
| Uses: | WB: Use at a concentration of 1 - 2 ug/ml. Detects a band of approximately 17 kDa. (Determined using a whole cell extract of mouse fibroblasts 3T3 cell line treated with sodium butyrate.)Indirect ELISA: Use at an assay dependent dilution. ICC: Use at an a |
| Application Summary: | ELISA, WB, ICC |
| General References: | General / background references:Fry CJ & Peterson CL Transcription. Unlocking the gates to gene expression. Science 295:1847-8 (2002). Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). |
| Notes Main: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
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