| CatalogCode: | NB120-12178 |
| ProductName: | Histone H3 Antibody |
| Product Description: | Rabbit Polyclonal anti-Histone H3 |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic acetylated [acetyl K9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3 conjugated to KLH. |
| Specificity: | Staining of (acetyl K9) histone H3 in immunoblotting is specifically inhibited with the immunizing peptide. There is no inhibition with non-acetylated histone H3 peptide. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse, Rat, Xenopus laevis, Cow, a wide range of other species, C. elegans, Chicken and Drosophilia melanogaster due to very high or perfect sequence identity across many species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | IF: Use at a dilution of 1/1000. (Determined by indirect immunofluorescence using the NIH3T3 mouse fibroblast cell line treated with sodium butyrate. WB: Use at a dilution of 1/1000. Detects a band of approximately 17 kDa. (Determined by immunoblotting using a using a whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate.)Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.We advise you to centrifuge this product vial before use. |
| Localization: | Nuclear |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 15mM Sodium AzideConstituents: 0.01M PBS. pH 7.4 |
| ListPrice: | 330 |
| AppSummary: | IF, WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti-H3/l antibody, anti-Histone H3 antibody |
| ProteinTarget: | Histone H3 (acetyl K9) |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Fry CJ & Peterson CL Transcription. Unlocking the gates to gene expression. Science 295:1847-8 (2002). Strahl BD & Allis CD The language of covalent histone modifications. Nature 403:41-5 (2000). Cheung P et al. Signaling to chromatin through histone modifications. Cell 103:263-71 (2000). Krebs JE et al. Global role for chromatin remodeling enzymes in mitotic gene expression. Cell 102:587-98 (2000). Kornberg RD & Lorch Y Twenty-five years of the nucleosome, fundamental particle of the eukaryote chromosome. Cell 98:285-94 (1999). Luger K & Richmond TJ The histone tails of the nucleosome. Curr Opin Genet Dev 8:140-6 (1998). Struhl K Histone acetylation and transcriptional regulatory mechanisms. Genes Dev 12:599-606 (1998). Zhang W et al. Essential and redundant functions of histone acetylation revealed by mutation of target lysines and loss of the Gcn5p acetyltransferase. EMBO J 17:3155-67 (1998). Kuo MH et al. Transcription-linked acetylation by Gcn5p of histones H3 and H4 at specific lysines. Nature 383:269-72 (1996). Sobel RE et al. Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4. Proc Natl Acad Sci U S A 92:1237-41 (1995). Turner BM & O'Neill LP Histone acetylation in chromatin and chromosomes. Semin Cell Biol 6:229-36 (1995). Thorne AW et al. Patterns of histone acetylation. Eur J Biochem 193:701-13 (1990). |
| NotesMain: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
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