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| Catalog Code: | NB120-12172 |
| Product Name: | HDAC4 Antibody |
| Product Description: | Rabbit Polyclonal anti-HDAC4 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: MSSQSHPDGLSGRDQPVEL, corresponding to amino acids 1-19 of Human HDAC4 conjugated to KLH with C-terminal added lysine. The corresponding sequence is identical in mouse and chicken. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Expected to cross-react with Chicken (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit IgG. |
| Uses: | IF: Use at a dilution of 1/250, this is determined by indirect immunofluorescence using HEK 293T cells expressing recombinant mouse HDAC4IP: Use at an assay dependent dilution. 10-20 ug of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 10<sup>7</sup> cells.WB: Use at an assay dependent dilution. Predicted molecular weight: 140 kDa. A minimum working dilution of 1/1000 is determined by blotting using whole extracts of mouse NIH-3T3 cells. A minimum working dilution of 1/500 is determined by blotting using whole extracts of rat brain. Additional weak bands may be detected when blotting using different extract preparations. Detection of HDAC4 by blotting is specifically inhibited with the immunizing peptide.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. HDAC4 is a class II histone deacetylase containing 1084 amino acid residues. HDAC4 has been shown to interact with NCoR. HDAC4 is a member of the class II mammalian histone deacetylases, which consists of 1084 amino acid residues. Its C terminal sequence is highly similar to the deacetylase domain of yeast HDA1. HDAC4, unlike other deacetylases, shuttles between the nucleus and cytoplasm in a process involving active nuclear export. Association of HDAC4 with 14-3-3 results in sequestration of HDAC 4 protein in the cytoplasm. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART. |
| Storage: | Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Preservative: 15mM Sodium Azide Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
| Application Summary: | immunofluorescence, immunoprecipitation, Western Blot |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-HA6116 antibody, anti-HD 4 antibody, anti-HD4 antibody, anti-HDAC 4 antibody, anti-HDAC A antibody, anti-HDACA antibody, anti-Histone Deacetylase 4 antibody, anti-Histone Deacetylase A antibody, anti-KIAA0288 antibody |
| Package Size: | 0.05 ml |
| Notes Main: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use |
| General References: | General / background references:Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999). |
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