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| Catalog Code: | NB120-12034 |
| Product Name: | LEF1 Antibody |
| Product Description: | Mouse Monoclonal anti-LEF1 (REMB1) |
| Clone: | REMB1 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Recombinant full length LEF1 (Human). |
| Specificity: | Recognizes the beta-Catenin Binding Domain of human LEF1 using immunoblotting (55-57 kDa). The antibody does not cross-react with TCF1 or TCF4. |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.05 mg protein G purified Mouse IgG. |
| Uses: | IF: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 5 ug/ml, this is recommended using whole extracts of human Jurkat cells or transiently expressed human LEF1 in whole cell lysates from Cos-1 cells. Predicted MW 55-57 kDa.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Lymphoid enhancer factor, LEF1, is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF1, TCF1, TCF3 and TCF4). These factors play crucial roles in WNT/Wingless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer. LEF1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF1 (for T cell Factor 1) for correct development of T lymphocytes in the thymus. LEF1 is a 399 amino acid protein. Alternative splice forms of LEF1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on immunoblots as small immunoreactive forms. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | 0.01M PBS. pH 7.5 |
| Application Summary: | immunofluorescence, Western Blot |
| Species Summary: | Hu, |
| Alternate Names: | anti-LEF1 antibody, anti-lymphoid enhancer-binding factor 1antibody, anti-HGNC:6551 antibody, anti-DKFZp586H0919 antibody, anti-TCF1ALPHAantibody, anti-lymphoid enhancer binding factor-1 antibody |
| Package Size: | 0.05 mg |
| Preservative: | 0.08% Sodium Azide |
| Notes Main: | Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| General References: | General / background references:Eastman Q & Grosschedl R Regulation of LEF-1/TCF transcription factors by Wnt and other signals. Curr Opin Cell Biol 11:233-40 (1999). |
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