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| Catalog Code: | NB120-11881 |
| Product Name: | CD95 Antibody |
| Product Description: | Mouse Monoclonal anti-CD95 (UT-1) |
| Clone: | UT-1 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | T cell lymphoma cells HUT-78. |
| Specificity: | The antibody UT-1 reacts with CD95 (Fas/APO-1), a 46 kDa glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. The antibody UT-1 does excellently induce Fas mediated apoptosis, similarly as CH11 antibody. |
| Cross Reactivity: | Cross-reacts with Human.Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse IgG. |
| Uses: | FACS: Use at an assay dependent dilution. FuncS: Use at an assay dependent dilution. The antibody induces apoptosis of CD95 expressing cell lines.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Type I membrane protein (isoform 1); Secreted (isoforms 2 to 6). |
| Background: | CD95, also known as FAS or APO1, is a 36 kDa cell surface type I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS PAGE. CD95 is a member of the TNF receptor family, which includes TNFR1, TNFR2, CD27, CD30 and CD40. Binding of CD95 Ligand to CD95 or crosslinking of CD95 by anti CD95 monoclonal antibodies leads to apoptosis of CD95 expressing cells. CD95 belongs to a subgroup of family members that have a death domain (DD) which contains 70 amino acids near the carboxyl terminal region of the molecule. The binding of adaptor molecules to this DD is responsible for transmitting the death signal for apoptosis. Stimulation of CD95 results in aggregation of its DD, leading to the recruitment of FADD and caspase 8 that together with the receptor form the death inducing signaling complex (DISC). CD95/CD95L is involved in the peripheral deletion of activated mature T cells at the end of the immune response and defects in this pathway predispose to autoimmune disorders. CD95 is also involved in killing of targets such as virus infected cells or cancer cells and killing of inflammatory cells at immune privileged sites. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgM |
| Host Name: | Mouse |
| Buffer: | PBS. pH 7.4 |
| Application Summary: | flow cytometry / FACS analysis, Other |
| Species Summary: | Hu, |
| Alternate Names: | anti-ALPS1A antibody, anti-APO1 cell surface antigen antibody, anti-Apoptosis antigen 1 antibody, anti-Apoptosis APO1 antigen 1 antibody, anti-Apoptosis mediating surface antigen FAS antibody, anti-APT1 antibody, anti-CD95 antibody, anti-FAS Antigen antibody, anti-FAS1 antibody, anti-FASL receptor antibody, anti-FASTM antibody, anti-TNFR6 antibody, anti-TNFRSF6 antibody, anti-Tumor necrosis factor receptor superfamily member 6 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 15mM Sodium Azide |
| Notes Main: | The antibody is provided as a sterile solution. |
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more information: | company product webpage |